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Microsomes isolated from the developing cotyledons of the seeds of the safflower varieties, very-high-linoleate, Gila and high-oleate, were capable of exchanging the acyl groups in acyl-CoA with the fatty acids in position 2 of phosphatidylcholine. The specificity of the 'acyl-exchange' towards the acyl moiety in acyl-CoA was selective in the order: oleate(More)
The separation of restriction endonuclease fragments of DNA on columns of Pharmacia PepRPC (C2/C18) has been studied. The effect of different concentrations of triethylammonium or tetrabutylammonium salts as ion-pairing reagents, as well as of physical parameters, such as flow-rate and sample load, has been investigated. With the use of triethylammonium(More)
Several prototypes of aromatic (Ar) and non-aromatic (NoAr) cation-exchange ligands suitable for capture of proteins from high conductivity (ca. 30 mS/cm) mobile phases were coupled to Sepharose 6 Fast Flow. These new prototypes of multi-modal cation-exchangers were found by screening a diverse library of multi-modal ligands and selecting cation-exchangers(More)
The hands of medical personnel are the chief vectors for transmission of antibiotic-resistant bacteria and probably serve as an important reservoir for antibiotic resistance genes in hospitals. In this survey we examined different reservoirs of the methicillin resistance gene, mecA, using a simplified PCR method. Samples (n = 151) were taken from the hands(More)
Microsomes of developing soya bean cotyledons transfer oleate from oleoyl-CoA to phosphatidylcholine (PC) by two different mechanisms: one in which oleate transfer is accompanied by the release of free CoA and another which results in the exchange of oleate from oleoyl-CoA for unsaturated 18-carbon fatty acids of PC. The acyl exchange can be demonstrated(More)
Several prototypes of multi-modal ligands suitable for the capture of negatively charged proteins from high conductivity (28 mS/cm) mobile phases were coupled to Sepharose 6 Fast Flow. These new prototypes of multi-modal anion-exchangers were found by screening a diverse library of multi-modal ligands and selecting anion-exchangers resulting in elution of(More)
Antibodies of type IgG may be divided into two classes, called lambda or kappa, depending on the type of light chain. We have identified a conserved pocket between the two domains CH1 and CL of human IgG kappa-Fab, which is not present in the lambda type. This pocket was used as a target docking site with the purpose of exploring the possibilities of(More)
Large numbers of samples can be efficiently processed through sequential reaction steps using a 96-pronged support that projects into individual microtiter wells. The support was constructed by creating a porous surface on a disposable polystyrene manifold, with avidin coupled to this greatly expanded surface. The shape and high binding capacity of the(More)
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