Gunja Gupta

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Recombinant proteins can be targeted to the Escherichia coli periplasm by fusing them to signal peptides. The popular pET vectors facilitate fusion of target proteins to the PelB signal. A systematic comparison of the PelB signal with native E. coli signal peptides for recombinant protein expression and periplasmic localization is not reported. We chose the(More)
Objective Eukaryotic cilia/flagella are dynamic microtubule (MT)based organelles. The ciliary axoneme is built from a radial scaffold of 9 MT doublets, plus a central MT pair, in the case of motile cilia. The axoneme is templated by a basal body (BB)/centriole derived from the mother centriole, in the case of animal cells, which presents sub-distal and(More)
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