Guillaume Lachapelle

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Hsp70 overexpression can protect cells from stress-induced apoptosis. Our previous observation that Hsp70 inhibits cytochrome c release in heat-stressed cells led us to examine events occurring upstream of mitochondrial disruption. In this study we examined the effects of heat shock on the proapoptotic Bcl-2 family member Bax because of its central role in(More)
We have developed new packaging cell lines (293SF-PacLV) that can produce lentiviral vectors (LVs) in serum-free suspension cultures. A cell line derived from 293SF cells, expressing the repressor (CymR) of the cumate switch and the reverse transactivator (rtTA2(S)-M2) of the tetracycline (Tet) switch, was established first. We next generated clones stably(More)
 The ORFs 5, 6 and 7, encoding for the three major structural proteins, GP5, M and N, of the IAF-Klop strain of PRRSV were cloned and expressed in 293 cells using replication-defective human type 5 adenoviral vectors (hAdVs). Although the M protein gene could be cloned into hAdVs and expressed constituvely in 293 cells under the control of the hCMV(More)
Inhibition of stress-induced apoptosis by the molecular chaperone protein Hsp70 is a contributing factor in tumorigenesis and suppression of this ability could increase the effectiveness of anti-tumor therapy. Tumor cells exist in an acidic environment and acute acidification can sensitize tumor cells to heat-induced cell death. However, the ability of(More)
Summary form only given. Loran-C, to be completed in 1990, is expected to provide coast-to-coast coverage over an area containing some 90% of Canada's population, while the Global Positioning System is expected to provide worldwide navigation coverage by 1992. The fundamental signal propagation and measurement characteristics of the 100 kHz Loran-C and(More)
The trends of specific technologies and their likely impact on navigation systems used in transportation during the next 25 years are described. Particular emphasis is placed on the Canadian situation. The research will contribute to Transport Canada objectives of exploring strategic technologies, fostering transportation research at universities, and(More)
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