Guilherme A. D. Dias

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Large volumes of plasma can be fractionated by the method of Cohn at low cost. However, liquid chromatography is superior in terms of the quality of the product obtained. In order to combine the advantages of each method, we developed an integrated method for the production of human albumin and immunoglobulin G (IgG). The cryoprecipitate was first removed(More)
The aim of the method described here is to remove hemoglobin, the major contaminant in the bovine plasma obtained from slaughterhouses, by adding a mixture of 19% cold ethanol and 0.6% chloroform, followed by fibrinogen and globulin precipitation by the Cohn method and nonspecific hemagglutinin by thermocoagulation. The experimental volume of bovine plasma(More)
We have modified a standard column chromatography method for the preparation of albumin from human plasma. The proposed method utilizes Sephadex G-25, euglobulin precipitation, DEAE-Sepharose, ethanol heat treatment and Sephacryl S-200 HR. The procedure differs from that normally used by the introduction of Schneider's ethanol/heat treatment step and the(More)
Immunoglobulin G (IgG) of excellent quality for intravenous use was obtained from the cryosupernatant of human plasma by a chromatographic method based on a mixture of ion-exchange, DEAE-Sepharose FF and arginine Sepharose 4B affinity chromatography and a final purification step by Sephacryl S-300 HR gel filtration. The yield of 10 experimental batches(More)
The mechanisms of antitumor effects associated with the Electro Current Therapy (ECT) treatment that consists of a DC current flow in the tumor region are not well understood. So, the aim of this paper is to present a Tumor Cellular Model to evaluate the potential profile developed across the cellular membrane, which separates the intracellular and(More)
The aim of this paper is to indicate the Electrostatic Treatment – EST (capacitive effect) as a cancer therapy and the studies that were be done by our research group using Electromagnetic Field Theory to explain the effects over the tumors. EST is a original, not expensive and non invasive cancer therapy which consists in to apply on a tumor continuos (dc)(More)
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