Gui-fang Shen

Publications15
h-index8
Citations422
Highly Influential Citations20
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Chloroplast Transformation in Oilseed Rape
The chloroplast transformation vector pNRAB carries two expression cassettes for the spectinomycin resistance gene aadA and the insect resistance gene cry1Aa10. The two cassettes are sited between… Expand
  • 132
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Recombination and expression of classical swine fever virus (CSFV) structural protein E2 gene in Chlamydomonas reinhardtii chroloplasts.
  • D. He, K. Qian, +4 authors H. Shao
  • Biology, Medicine
  • Colloids and surfaces. B, Biointerfaces
  • 15 March 2007
The expression of classical swine fever virus (CSFV) structural protein E2 in different vectors, which has been shown to carry critical epitopes, has been established. Here, we reported a… Expand
  • 78
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Expression of human soluble TRAIL in Chlamydomonas reinhardtii chloroplast
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induces selectively apoptosis in various tumor cells and virus-infected cells, but rarely in normal cells. A chloroplast expression… Expand
  • 45
  • 3
The Klebsiella pneumoniae nitrogenase Fe protein gene (nifH) functionally substitutes for the chlL gene in Chlamydomonas reinhardtii.
The entire coding region of chlL, an essential chloroplast gene required for chlorophyll biosynthesis in the dark in Chlamydomonas reinhardtii, was precisely replaced by either the Klebsiella… Expand
  • 51
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Stable expression of foot-and-mouth disease virus protein VP1 fused with cholera toxin B subunit in the potato (Solanum tuberosum).
  • D. He, K. Qian, +4 authors H. Shao
  • Biology, Medicine
  • Colloids and surfaces. B, Biointerfaces
  • 1 April 2007
The expression vector, pBI121CTBVP1, containing the fusion of the foot and mouth disease virus (FMDV) VP1 gene and the cholera toxin B subunit (CTB) gene was constructed by fused PCR and transferred… Expand
  • 16
  • 2
The expression of classical swine fever virus structural protein E2 gene in tobacco chloroplasts for applying chloroplasts as bioreactors.
It has been reported that genes encoding antigens of bacterial and viral pathogens can be expressed in plants and are shown to induce protection antibodies. The structural protein E2 of classical… Expand
  • 17
  • 1
Survival of human metallothionein-2 transplastomic Chlamydomonas reinhardtii to ultraviolet B exposure.
Solar ultraviolet (UV) radiation has a great influence on green organisms, especially plankton like Chlamydomonas. A human metallothionein-2 gene, which is generally considered to have an… Expand
  • 33
Nif gene transfer and expression in chloroplasts: Prospects and problems
The engineering of plants capable of fixing their own nitrogen is an extremely complex task, requiring the co-ordinated and regulated expression of 16 nif genes in an appropriate cellular location.… Expand
  • 39
Establishment of a Gene Expression System in Rice Chloroplast and Obtainment of PPT-Resistant Rice Plants
Abstract In contrast to the situation of random integration of foreign genes in nuclear transformation, the introduction of genes via chloroplast genetic engineering is characterized by site-specific… Expand
  • 6
[Antiulcer effects and mechanism study of Veronicastrum axillare on ethanol induced gastric ulcer rats].
OBJECTIVE To study the antiulcer effects and the mechanism of Veronicastrum axillare (Sieb. et Zucc) Yamazaki (VAY) on ethanol induced gastric ulcer rats. METHODS Totally 48 healthy SD rats were… Expand
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