Gui-fang Fu

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OBJECTIVE Studying the gene expression profiling of different stage hairy root of Salvia miltiorrhiza, in order to find functional genes. METHOD The contents of second metabolites were determined by HPLC and gene expression profiling was detected by cDNA microarray. cDNA labeled with a fluorescent dye (Cy5 and Cy3-dCTP) was produced by Eberwine's linear(More)
OBJECTIVE Establishing cDNA microarray, in order to study functional genomics of Salvia miltiorrhiza. METHOD Total RNA samples were prepared from S. miltiorrhiza roots using a modified CTAB method. mRNA was isolated by Quichprep Micro mRNA Purification Kit from Pharmacia. Then cDNA was synthesized and cloned into the EcoRI-XhoI sites of the ZAP Express(More)
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