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In this study a three step culture system, 2D-3D sequential culture in vitro and further implantation in vivo was developed to induce human embryonic stem cells (hESCs) into cartilage like tissues. Five-day-old embryoid bodies were plated for chondrogenic induction for 27 days (step1), then the cells were suspended in alginate and seeded onto(More)
OBJECTIVE The purpose of this study was to assess the effects of hyperbaric oxygen (HBO) and near-infrared light therapy on wound healing. BACKGROUND DATA Light-emitting diodes (LED), originally developed for NASA plant growth experiments in space show promise for delivering light deep into tissues of the body to promote wound healing and human tissue(More)
Flexible electronics is an emerging and promising technology for next generation of optoelectronic devices. Herein, hierarchical three-dimensional ZnCo(2)O(4) nanowire arrays/carbon cloth composites were synthesized as high performance binder-free anodes for Li-ion battery with the features of high reversible capacity of 1300-1400 mAh g(-1) and excellent(More)
OBJECTIVE To study expression of cystic fibrosis transmembrane conductance regulator (CFTR) in human endometrium. METHODS The expression of CFTR mRNA and protein from 50 samples of normal cyclic human endometrium was examined by in situ hybridization, immunohistochemistry and Western blotting respectively. RESULTS CFTR mRNA and protein expressions were(More)
Human embryonic stem (hES) cells with the capacity of self-renewal and multilineage differentiation are promising sources for generation of pancreatic islet cells for cell replacement therapy in diabetes. Here we induced hES cells into insulin-producing cells (IPCs) in a stepwise process which recapitulated islet organogenesis by directing cells through the(More)
BACKGROUND Vitrification is assumed to be a promising method to cryopreserve human oocytes but still needs optimization. In this study, rabbit oocytes (fertilized by ICSI) were vitrified with cryoloops, and the effect of three different cryopreservation protocols on spindle configuration and embryo quality was assessed. METHODS Metaphase II rabbit oocytes(More)
Islet-like cells derived from embryonic stem (ES) cells may be a promising therapeutic option for future diabetes treatment. Here, we demonstrated a five-stage protocol with adding exendin-4 instead of nicotinamide finally could generate islet-like cells from human embryonic stem (ES) cells. Immunofluorescence analysis revealed a high percentage of(More)
Rice is known as an ammonium (NH4 +)-tolerant species. Nevertheless, rice can suffer NH4 + toxicity, and excessive use of nitrogen (N) fertilizer has raised NH4 + in many paddy soils to levels that reduce vegetative biomass and yield. Examining whether thresholds of NH4 + toxicity in rice are related to nitrogen-use efficiency (NUE) is the aim of this(More)
Previous studies have shown that the aromatase activity in human endometrial stromal cells was stimulated by progestin and enhanced by estrogen and forskolin (Fk), an agent that stimulates the accumulation of intracellular cAMP. Present study was undertaken to investigate whether any peptide hormone would affect endometrial aromatase activity. Stromal cells(More)
The effects of relaxin (RLX), forskolin (Fk), and 4-(3-butoxy-4-methoxybenzyl)-2-imidazolidinone (Ro, a phophodeisterase inhibitor) on the accumulation of cyclic adenosine 3',5'-monophosphate (cAMP) in human endometrial glandular epithelial cells were studied. Epithelial glands were isolated from the endometrium by digesting the viable tissue fragments with(More)