Guenter Adam

Learn More
Ribgrass mosaic virus (RMV), turnip vein-clearing virus (TVCV) and Youcai mosaic virus (YoMV; formerly designated as oilseed rape mosaic virus; ORMV) belong to the genus Tobamovirus and are arranged in one out of three subgroups because of their common host range, serological cross-reactivity and amino acid composition of their coat proteins. The recently(More)
A procedure involving reverse transcription followed by the polymerase chain reaction (RT-PCR) using a single primer pair was developed for the detection of five tobamovirus species which are related serologically. Either with a subsequent restriction enzyme analysis (RT-PCR-RFLP) or with a RT-PCR using species specific primers the five species can be(More)
The Arabidopsis CPD gene encodes a cytochrome P450 steroid side-chain hydroxylase (CYP90) that plays an essential role in the biosynthesis of the plant hormone brassinolide. Expression of the CPD gene is confined to cotyledons and leaf primordia in etiolated seedlings and detectable in the adaxial parenchyma of expanding leaves in light-grown plants.(More)
Recent discoveries from brassinosteroid-deficient mutants led to the recognition that plants, like animals, use steroids to regulate their growth and development. We describe the characterization of one member of a Brassica napus sulfotransferase gene family coding for an enzyme that catalyzes the O-sulfonation of brassinosteroids and of mammalian(More)
Double-stranded RNA (dsRNA) is in many cases the only available template for molecular and diagnostic studies of RNA viruses. A novel mycovirus with a five dsRNAs segmented-genome served as a model system for the amplification and cloning of dsRNA segments using several PCR-based methods. Sequences obtained by the classical method; random PCR (rPCR) with a(More)
Ten Fusarium graminearum isolates from China were screened for dsRNA mycoviruses. Five dsRNAs (2.4 to 3.5 kbp) were purified from isolate China 9, cloned, and sequenced. BLAST analysis showed that the proteins encoded by dsRNA1 possess motifs that are conserved in RNA-dependent RNA polymerases, dsRNA2 resembles the hypothetical protein encoded by dsRNA3 of(More)
A virus isolate from Pelargonium spp., provisionally designated UPEV ( ${\underline {\rm u}}{\rm nknown} \ {\underline {\rm pe}} {\rm largonium} \ {\underline {\rm v}}{\rm irus}$ ), had isometric particles 31–33 nm in diameter, with a granular surface structure similar to that of viruses in three genera of family Tombusviridae. Immunoelectron microscopy(More)
Hormônios vegetais brassinosteroídicos – estrutura, bioatividade e aplicações: Os brassinosteróides são uma nova classe de hormônios vegetais com estrutura esteroídica polioxigenada, dotados de pronunciada atividade reguladora do crescimento vegetal. Esta revisão cobre sua ocorrência natural, os métodos biológicos e cromatográficos para sua detecção, sua(More)
We examined the resistance phenotype of a large number of transgenic tobacco plants originating from 12 commercial (Nicotiana tabacum) cultivars expressing the sense form of the nucleoprotein (N) gene of L3, a Bulgarian isolate of tomato spotted wilt virus (TSWV). The analysis revealed that transgenic plants are completely protected against the homologous(More)