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Ovalbumin and lysozyme are two main proteins in hen egg white with the isoelectric points of 4.8 and 11, respectively. Herein we report the manufacture of stable, narrowly distributed nanogels (hydrodynamic radius about 100 nm) using a novel and convenient method: ovalbumin and lysozyme solutions were mixed at pH 5.3, the mixture solution was adjusted to pH(More)
The denaturation of collagen solution in acetic acid has been investigated by using ultra-sensitive differential scanning calorimetry (US-DSC), circular dichroism (CD), and laser light scattering (LLS). US-DSC measurements reveal that the collagen exhibits a bimodal transition, i.e., there exists a shoulder transition before the major transition. Such a(More)
Zonula occludens proteins (ZOs), including ZO1/2/3, are tight junction-associated proteins. Each of them contains three PDZ domains. It has been demonstrated that ZO1 can form either homodimers or heterodimers with ZO2 or ZO3 through the second PDZ domain. However, the underlying structural basis is not well understood. In this study, the solution structure(More)
The fabrication of hollow microspheres to encapsulate functional molecules, such as drugs, insecticides, and proteins, is of ever-increasing importance. Many chemical and physicochemical methods have been tested for various specific encapsulations, but most of them have not been developed into an industrial process. In this work, we present a(More)
Copolymerization of methyl methacrylate (MMA) and acrylate terminated poly(ethylene oxide-co-ethylene carbonate) (PEOC) macromonomer yields graft copolymer with PEOC side chains. By use of quartz crystal microbalance with dissipation (QCM-D), we have investigated the adsorption of proteins on the surface constructed by the copolymer in seawater. It shows(More)
The lower critical solution temperature (LCST) behaviors of random and segmented copolymers of N-isopropylacrylamide (NIPAM) and acrylic acid (AA) prepared in dioxane and water have been investigated by using ultrasensitive microcalorimetry (US-DSC). The introduction of AA increases the LCST of the former but slightly affects that of the latter. When the(More)
Poly(sodium styrenesulfonate) (PSSNa) chains have been grafted onto a SiO(2)-coated resonator surface. The conformational changes of grafted chains have been investigated using a quartz crystal microbalance with dissipation (QCM-D) in the presence of monovalent or multivalent salts as a function of ionic strength. In the case of monovalent counterions, the(More)
Polyurethane (PU) with zwitterionic side chains has been prepared to resist nonspecific adsorption of proteins. First, dihydroxy-terminated poly(2-(dimethylamino)ethyl methacrylate) (PDEM(OH)(2)) is synthesized by free radical polymerization with 3-mercapto-1,2-propanediol as the chain transfer agent, which polyadds with diisocyanate to yield a PU with PDEM(More)
Two kinds of thermally sensitive oppositely charged polymeric microgels, poly(N-isopropylacrylamide-co-sodium acrylate) (NIPAM-co-SA) and poly(N-isopropylacrylamide-co-vinylbenzyl trimethylammonium chloride) (NIPAM-co-VT), have been prepared by dispersion polymerization. Their temperature-induced aggregation was studied by use of laser light scattering. In(More)