Guangsheng Luo

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A new and simple method has been proposed to prepare magnetic Fe(3)O(4)-chitosan (CS) nanoparticles by cross-linking with sodium tripolyphosphate (TPP), precipitation with NaOH and oxidation with O(2) in hydrochloric acid aqueous phase containing CS and Fe(OH)(2), and these magnetic CS nanoparticles were used to immobilize lipase. The effects on the(More)
The influences of pore diameter and cross-linking method on the immobilization efficiency of Candida rugosa lipase (CRL) in a mesoporous material have been investigated. Five kinds of SBA-15 with different pore-sizes (6.8nm, 9.1nm, 13.2nm, 15.6nm and 22.4nm) were chosen as the carrier. The one with pore diameter of 15.6nm was proved to be a more suitable(More)
In this study, macro-mesoporous silica spheres were prepared with a micro-device and used as the support for the immobilization of penicillin G acylase (PGA). To measure the enzymatic activity, the silica spheres with immobilized PGA were placed into a packed-bed reactor, in which the hydrolysis of penicillin G was carried out. The influences of the(More)
When chitosan nanoparticles were prepared in a water-in-oil (W/O) microemulsion by using 2% (wt) acetic acid (HAc) and 30% (wt) tri-n-octylamine (TOA) as solvent and precipitant, respectively, particle diameters of 7 nm were observed and the particles formed ovoid shaped aggregates. Using 0.05% HCl and 5.0M NaOH as solvent and precipitant produced(More)
The adsorption characteristics of BSA onto the magnetic chitosan nanoparticles have been investigated in this paper. The magnetic chitosan nanoparticles were prepared by adding the basic precipitant of NaOH solution into a W/O microemulsion system. The morphology of magnetic chitosan nanoparticles was observed by transmission electron microscope (TEM). It(More)
Mesostructured cellular foams (MCFs) are suitable for biomolecular immobilization because of their relatively large-pore diameter and pore volume. Penicillin G acylase (PGA) was immobilized on aminopropyl-functionalized MCFs through Schiff base reaction. It is shown that PGA could be fixed more firmly through the covalent immobilization on(More)
The effects of alcohol treatment on the activity and loading amount of Candida rugosa lipase (CRL), Candida Antarctica lipase B (CALB) and Porcine Pancreas lipase (PPL) immobilized on methyl-modified silica aerogels were investigated, and the fluorescent analysis was used to explore the change of lipase hydrophobicity in aqueous solution caused by alcohols.(More)
OBJECTIVE To determine taxol in aril of Torreya grandis cv. merrilli. METHODS Extracts from Torreya grandis cv. merrilli were obtained by cold extraction and soxhlet extraction with ethanol, butyl acetate and chloroform as solvent. The content of taxol in extracts was determined by HPLC, and whether components similar with taxol existed in which was(More)
This article describes a simple method for the fabrication of microscale polymer tubes. A double co-axial microchannel device was designed and fabricated. Liquid/liquid/liquid multiphase co-laminar flows were realized in a microchannel by choosing working systems. Three kinds of polymeric solutions were selected as the middle phase while a(More)
To develop a green, cost-efficient and robust peroxidase mimic, micro/nano hierarchical morphology (for ease of separation and reuse), relative chemically stable composition (for ease of storage) and stable crystal structure (for long-term stability) are highly desired. Herein, using phosphoric acid as a chelating ligand to control the release of iron ions,(More)