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An aerobic, Gram-negative bacterial strain, designated CTN-1(T), capable of degrading chlorothalonil was isolated from a long-term chlorothalonil-contaminated soil in China, and was subjected to a polyphasic taxonomic investigation. Strain CTN-1(T) grew at 15-37 °C (optimum 28-30 °C) and at pH 6.0-9.0 (optimum pH 7.0-7.5). The G+C content of the total DNA(More)
The reaction cycle of HECT domain ubiquitin ligases consists of three steps: 1) binding of an E2 protein, 2) transfer of ubiquitin from E2 to the HECT domain, and 3) transfer of ubiquitin to the substrate. We report the identification of a determinant that is specifically required for the last step of this cycle, a phenylalanine residue located four amino(More)
Dehalogenases play key roles in the detoxification of halogenated aromatics. Interestingly, only one hydrolytic dehalogenase for halogenated aromatics, 4-chlorobenzoyl-coenzyme A (CoA) dehalogenase, has been reported. Here, we characterize another novel hydrolytic dehalogenase for a halogenated aromatic compound from the 2,4,5,6-tetrachloroisophthalonitrile(More)
OBJECTIVE To assess the feasibility of visualizing hand and foot tendon anatomy and disorders by Gemstone Spectral Imaging (GSI) high-definition CT (HDCT). MATERIALS AND METHODS Thirty-five patients who suffered from hand or foot pain were scanned with GSI mode HDCT and MRI. Spectrum analysis was used to select the monochromatic images that provide the(More)
Recent studies in mice have shown that resveratrol can protect the liver from fat accumulation induced by high fat diet. However, the exact mechanism is largely unknown. To explore the possible mechanism, we investigated the anti-lipogenic effect of resveratrol in vitro model. Oil Red O staining revealed that resveratrol could significantly ameliorate the(More)
Dopamine-coordinated photoactive TiO(2) nanoporous films with a wide excitation range of light in the visible region (up to 580 nm) were prepared and used for sensitive detection of NADH. Colloidal TiO(2) was firstly covered on an indium-tin oxide (ITO) electrode surface and sintered at 450 degrees C to form a nanoporous TiO(2) film, then the electrode was(More)
A Gram-stain-negative, non-motile, rod-shaped bacterial strain, JW-64-1(T), capable of degrading methamidophos was isolated from a methamidophos-manufacturing factory in China, and was subjected to a polyphasic taxonomic investigation. Strain JW-64-1(T) produced circular, smooth, transparent, yellow-colored colonies (1.0-2.0 mm) on LB agar after 2 days(More)
The objective of this study was to evaluate the possible association between the CD14-159 polymorphism and adult asthma in the Chinese population. A total of 188 asthmatic patients and 60 healthy adults were enrolled in the present study, and the CD14-159 polymorphism was genotyped using the polymerase chain reaction-restriction fragment length polymorphism(More)
Methamidophos is one of the most widely used organophosphorus insecticides usually detectable in the environment. A facultative methylotroph, Hyphomicrobium sp. MAP-1, capable of high efficiently degrading methamidophos, was isolated from methamidophos-contaminated soil in China. It was found that the addition of methanol significantly promoted the growth(More)
An AAP-degrading bacterium, AAP-7, was isolated from AAP-polluted soil. AAP-7 was identified as Pseudoxanthomonas sp. on the basis of the comparative analysis of 16S rDNA sequences. The strain was able to transformate more than 80% AAP by means of co-metabolism and degraded AAP via hydrolysis or demethylation to form(More)