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To compare microbial functional diversity in different oil-contaminated fields and to know the effects of oil contaminant and environmental factors, soil samples were taken from typical oil-contaminated fields located in five geographic regions of China. GeoChip, a high-throughput functional gene array, was used to evaluate the microbial functional genes(More)
In environmental microbiology, the most commonly used methods of bacterial DNA transfer are conjugation and electroporation. However, conjugation requires physical contact and cell-pilus-cell interactions; electroporation requires low-ionic strength medium and high voltage. These limitations have hampered broad applications of bacterial DNA delivery. We(More)
High-density functional gene arrays have become a powerful tool for environmental microbial detection and characterization. However, microarray data normalization and comparison for this type of microarray remain a challenge in environmental microbiology studies because some commonly used normalization methods (e.g., genomic DNA) for the study of pure(More)
Four pilot-scale test mesocosms were conducted for the remediation of organochlorine pesticides (OCPs)-contaminated aged soil. The results indicate that the effects on degradation of hexachlorocyclohexanes (HCHs) and dichlorodiphenyltrichloroethanes (DDTs) were in the following order: nutrients/plant bioaugmentation (81.18 % for HCHs; 85.4 % for DDTs) >(More)
Based on column experiments, the oxidative degradations of some chlorinated hydrocarbons under three less-reduced redox conditions were investigated. The results showed that in the presence of nitrate and manganese oxide, 1,2-dechloroethane (1,2-DCA) and vinyl chloride (VC) could be oxidized. The transformation rates of 1,2-DCA under denitrification and(More)
Congestive heart failure (CHF) is a major cause of hospitalizations, morbidity, and mortality in Western societies. In addition to optimal medical and device therapy, exercise training is an important adjunct treatment option for CHF patients. MicroRNAs (miRNAs, miRs) participate in a variety of physiological and pathological processes. Dynamic regulation(More)
In a study to optimize bacterial whole cell biosensors (bioreporters) for the detection of environmental contaminants, we constructed a toxicity sensing strain Acinetobacter baylyi ADP1_recA_lux. The ADP1_recA_lux is a chromosomally based bioreporter which makes the sensing system stable and negates the need for antibiotics to maintain the trait. The(More)
A whole-cell bacterial bioreporter Acinetobacter baylyi strain ADP1_recA_lux that responds to genotoxins was employed to directly assess the adverse effects of the bioavailable fraction of mitomycin C (MMC), benzo[a]pyrene (BaP), chromium (VI) and lead (II) in amended soils and soil samples from two fragile areas in China without soil pre-treatment. The(More)
Ozonation with a subsequent biodegradation treatment was performed to remove recalcitrant organic compounds from long-term weathered crude oil contaminated soil. Samples were analyzed by GC/MS and column chromatography to monitor changes in crude oil composition. A functional gene array was used to examine microbial community dynamics. After a 6h ozonation(More)
Successful site remediation is critically based on a comprehensive understanding of distribution of contaminants, soil physico-chemical and microbial properties in oil contaminated sites. One hundred and ten topsoils were sampled from seven typical oil fields in different geoclimate regions across north to south China to investigate the spatial variances of(More)