Guang-Xiang Qian

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OBJECTIVE To detect circulating hepatocellular carcinoma by demonstrating hepatocellular carcinoma cells or hepatocyte-associated mRNA in the nuclear cell component of peripheral blood (PBL). METHODS Peripheral blood (5 ml samples were obtained from 93 patients with hepatocellular carcinoma (HCC) and from 33 control subjects (9 with liver cirrhosis after(More)
OBJECTIVES To detect blood AFPmRNA in the nude mice bearing with human hepatocellular carcinoma (HCC) by using nested reverse transcriptase polymerase chain reaction (nested RT-PCR), and assess its significance in HCC distant metastasis. METHOD We detected 20 blood samples from the nude mice bearing with human HCC by nested RT-PCR to find out AFPmRNA. (More)
OBJECTIVE To study the effects of percutaneous ethanol injection (PEI) in treatment of hepatocellular carcinoma (HCC). METHODS Blood samples were taken from 9 HCC patients and detected by nested RT-PCR to find out AFPmRNA after PEI treatment 3-5 times within 2 weeks. RESULTS Three (44.44%) of 4 patients with AFPmRNA positive turned to be negative after(More)
OBJECTIVES To detect the expression of human alpha-fetoprotein mRNA (AFPmRNA) in peripheral blood (PBL) of recurrent hepatocellular carcinoma (HCC) by using nested reverse transcriptase polymerase chain reaction (nested RT-PCR) and to discuss relations between AFPmRNA and recurrent HCC. METHODS Five ml peripheral blood samples were obtained from 19(More)
OBJECTIVE To investigate whether apoptotic cell death is involved in liver xenograft rejection and the molecular mechanism of apoptosis. METHODS After hamster-to-rat orthotopic liver transplantation, apoptosis in the xenograft was observed histologically and by in situ end-labelling of fragmented DNA. CD8 antigen, perforin, Fas-L and TGF-beta1 were(More)
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