Gretchen L Oshop

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In this report, we show that dimethylsulfoxide (DMSO) enhances liposome-mediated transfection of nucleic acid in chicken macrophage cells and that this could be exploited for the transcutaneous delivery of naked DNA through the intact skin of chickens. We found that DMSO enhanced transfection efficiencies of lipofectamine and polyethyleneimine in HD-11(More)
Inoculation of naked DNA represents a novel approach to vaccine and immune therapeutic development. DNA vaccines or genetic immunization offers several advantages over the conventional vaccines for specific immune activation. Although a large number of vaccines have been made and are being used in the poultry industry, there have been no major advances in(More)
We have developed a simple method of transfecting avian embryos in ovo with various plasmid vectors that results in protein expression in the embryo. Using the chloramphenicol acetyl transferease (CAT) reporter gene, we were able to show that transfecting avian embryos with a plasmid/neutral lipid/dimethylsulfoxide (DMSO) mixture delivered to the air cell,(More)
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