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FK506 (tacrolimus) is a secondary metabolite with a potent immunosuppressive activity, currently registered for use as immunosuppressant after organ transplantation. FK506 and FK520 are biogenetically related natural products that are synthesized by combined polyketide synthase/nonribosomal peptide synthetase systems. The entire gene cluster for… (More)
FK506 (Tacrolimus) is an important immunosuppressant, produced by industrial biosynthetic processes using various Streptomyces species. Considering the complex structure of FK506, it is reasonable to expect complex regulatory networks controlling its biosynthesis. Regulatory elements, present in gene clusters can have a profound influence on the final yield… (More)
We report the draft genome of Streptomyces rimosus (ATCC 10970), a soil isolate that produces oxytetracycline, a commercially important and clinically useful antibiotic.
Trypanosoma cruzi, the parasite causing Chagas disease, contains a number of proteolytic enzymes. The recent completion of the genome sequence of the T. cruzi CL Brener clone suggests the presence of 70 cysteine peptidases, 40 serine peptidases (none of them from the chymotrypsin family), about 250 metallopeptidases (most leishmanolysin homologues), 25… (More)
Streptomyces rapamycinicus strain NRRL 5491 produces the important drug rapamycin. It has a large genome of 12.7 Mb, of which over 3 Mb consists of 48 secondary metabolite biosynthesis clusters.
A comparison between weak form meshless local Petrov-Galerkin method (MLPG) and strong form meshless diffuse approximate method (DAM) is performed for the diffusion equation in two dimensions. The shape functions are in both methods obtained by moving least squares (MLS) approximation with the polynomial weight function of the fourth order on the local… (More)
BACKGROUND Erythromycin is a medically important antibiotic, biosynthesized by the actinomycete Saccharopolyspora erythraea. Genes encoding erythromycin biosynthesis are organized in a gene cluster, spanning over 60 kbp of DNA. Most often, gene clusters encoding biosynthesis of secondary metabolites contain regulatory genes. In contrast, the erythromycin… (More)
The performance of the parallel implementation of the local meshless numerical method in solving system of coupled partial differential equations is explored. Presented numerical approach makes the computation convenient for parallel implementation using OpenMP based parallelization. The numerical experiments are performed on the de Vahl Davis natural… (More)
The high G+C content and large genome size make the sequencing and assembly of Streptomyces genomes more difficult than for other bacteria. Many pharmaceutically important natural products are synthesized by modular polyketide synthases (PKSs) and nonribosomal peptide synthetases (NRPSs). The analysis of such gene clusters is difficult if the genome… (More)
This paper introduces an effective H-adaptive upgrade to solution of the transport phenomena by the novel Local Radial Basis Function Collocation Method (LRBFCM). The transport variable is represented on overlapping 5-noded influence-domains through collocation by using multiquadrics Radial Basis Functions (RBF). The involved first and second derivatives of… (More)