Gregg B. Nyberg

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Metabolic flux analysis is a useful tool for unraveling relationships between metabolism and cell function. Material balancing can be used to provide estimates of major metabolic pathway fluxes, provided all significant metabolite uptake and production rates are measured. Potential sources of metabolizable material in many serum-free media formulations are(More)
The application of a stoichiometric medium design approach was studied in fed-batch cultivation of Chinese hamster ovary (CHO) cells. A serum-free medium containing a very low protein concentration (2 mg/L insulin) was developed. A supplemental medium was formulated according to the stoichiometric equation governing cell growth using cell composition(More)
....................................... 3 ACKNOWLEDGMENTS .......................................................................................................... 5 TABLE OF CONTENTS .............................. 7 LIST OF FIGURES ............................................................................................... 11 LIST OF TABLES(More)
Asparagine linked (N-linked) glycosylation is an important modification of recombinant proteins, because the attached oligosaccharide chains can significantly alter protein properties. Potential glycosylation sites are not always occupied with oligosaccharide, and site occupancy can change with the culture environment. To investigate the relationship(More)
Based on a detailed metabolic network of CHO-320 cells built using available information gathered from published reports, the flux distribution can be evaluated using tools of positive linear algebra (in particular, the algorithm METATOOL devised in T. Pfeiffer and Schuster (1999); S. Schuster (1999)), and to study the influence of the interconnection level(More)
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