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While a great deal of knowledge on the mechanisms of steroid hormone regulated gene expression now exists, specific information relating to androgens is lacking. A number of experimental systems have been developed and show promise as models for molecular studies of androgen regulation. Further development of these models must address the issue of whether(More)
Ornithine decarboxylase, the first enzyme of the polyamine biosynthetic pathway, is induced by androgens in the mouse kidney. We have isolated from a kidney cDNA clone bank two plasmids, pODC1440 and pODC934 , that contain different cDNA inserts corresponding to ornithine decarboxylase mRNA. Identification was based upon the ability of plasmid-specific(More)
The concentrations of several mRNAs in mouse kidney increase in response to testosterone. To determine if the increases are generated at the level of gene transcription, we have assayed transcription rates for several androgen-inducible mRNAs in kidney nuclei in vitro. No significant changes were found in the synthesis of three mRNAs whose concentrations(More)
A regulatory locus, Gus-r, determines the rate and extent of androgen inducibility of beta-glucuronidase in mouse kidney epithelial cells. The kinetics of induction are strikingly similar when enzyme concentration, rates of enzyme synthesis, and beta-glucuronidase mRNA are measured. After an initial lag period the accumulation of mRNA activity obeys simple(More)
Studies on hormonal action frequently focus on a single hormone. In intact animals, however, genes may respond to the balance of multiple hormones. Therefore, we have studied the mutual influence of sex steroids on eight genes previously known to be testosterone-responsive in kidneys of mice. A variety of responses to estrogen were recorded. Effects(More)
Measurements of enzyme activity, rates of protein synthesis, and mRNA activity suggest that the induction of beta-glucuronidase in mouse kidney in response to androgen is regulated at a pretranslational level. Following an initial lag period, the rate and extent of induction follow the rules of simple turnover kinetics and can be described in terms of a(More)
The three alcohol dehydrogenase genes in the mouse are subject to developmental, hormonal, and genetic control as revealed by variation in expression among inbred strains. The primary purpose of this study was to determine the mechanism by which androgen regulates the expression of the Adh-1 gene in kidney. In addition, the fold-induction in several inbred(More)
A regulatory locus in a higher organism has been shown to control a specific messenger RNA activity. The Gur locus in mice regulates the production of kidney beta-glucuronidase messenger RNA activity after induction of the beta-glucuronidase structural gene, Gus, by testosterone. beta-Glucuronidase messenger RNA was assayed by its ability to direct the(More)
Clones containing sequences corresponding to testosterone-inducible RNAs of mouse kidney have been identified within a cDNA clone bank prepared from size-fractionated poly(A)-containing kidney RNA. A novel screening method was employed to specifically detect such sequences. One of these, pMK908, containing a 1.2 kilobase pair insert, was studied in detail.(More)