Grant Daggard

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Immature embryo-derived calli of spring wheat (Triticum aestivum L.) cv Veery5 were transformed using Agrobacterium tumefaciens strain LBA4404 carrying either binary vector pHK22 or superbinary vector pHK21, the latter carrying an extra set of vir genes – vir B, -C and -G. In both cases, transient β-glucuronidase (GUS) expression ranging from 35–63% was(More)
In response to the rapid development of DNA Microarray technology, many classification methods have been used for Microarray classification. SVMs, decision trees, Bagging, Boosting and Random Forest are commonly used methods. In this paper, we conduct experimental comparison of LibSVMs, C4.5, BaggingC4.5, AdaBoostingC4.5, and Random Forest on seven(More)
A screening was conducted to study the allelopathic potential of Australian-held accessions of Triticum speltoides. Of 26 accessions, four were found to inhibit root growth in the indicator species, lettuce (Lactuca sativa). The methanol leaf extracts of these accessions significantly reduced the root length of wild oat (Avena spp.). In all but one case,(More)
We investigate the idea of using diversified multiple trees for Microarray data classification. We propose an algorithm of Maximally Diversified Multiple Trees (MDMT), which makes use of a set of unique trees in the decision committee. We compare MDMT with some well-known ensemble methods, namely AdaBoost, Bagging, and Random Forests. We also compare MDMT(More)
The purpose of the study was to determine the epidemiological relationships in three unrelated cases of neonatal late-onset Group B streptococcal (GBS) disease and maternal breast-milk infection with GBS. All deliveries were by cesarean section; case 1 was at term, and cases 2 and 3 were at 32- and 33-wk gestation, respectively. Case 1 relates to a mother(More)
In recent years, the rapid development of DNA Microarray technology has made it possible for scientists to monitor the expression level of thousands of genes in a single experiment. As a new technology, Microarray data presents some fresh challenges to scientists since Microarray data contains a large number of genes (around tens thousands) with a small(More)
AIMS The purpose of this study was to determine if DNA polymorphisms generated by RAPD-PCR could be used to characterize Group B streptococci (GBS) for epidemiological purposes. METHODS AND RESULTS 30 unrelated, previously serotyped strains were analysed by RAPD-PCR using two 10-mer primers (5' TGCGAGAGTC 3' and 5' AGAGGGCACA 3'). Both primers generated(More)
Levels of transcripts produced by a heat shock protein 70 (hsp70)-antisense white transgene in Drosophila were measured after single and multiple heat shocks to determine whether the hsp70 promoter could produce sustained high levels of transgene transcripts. A single heat shock resulted in typical highly inducible levels of RNA, but the amount of antisense(More)
Plasma anticardiolipin antibody (ACA) was measured in 83 patients having coronary artery bypass graft surgery and results were correlated with the incidence of early (1-2 weeks) and late (12 months) graft occlusion, as judged by angiography. There was an association between preoperative ACA level and the incidence of late graft occlusion in relation to both(More)
The immunogenicity and protective efficacy of a DNA vaccine encoding a genetically inactivated S1 domain of pertussis toxin was evaluated using a murine respiratory challenge model of Bordetella pertussis infection. It was found that mice immunized via the intramuscular route elicited a purely cell-mediated immune response to the DNA vaccine, with high(More)