Grainne Byrne

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Surface activity and sensitivity to inhibition from phospholipase A2 (PLA2), lysophosphatidylcholine (LPC), and serum albumin were studied for a synthetic C16:0 diether phosphonolipid (DEPN-8) combined with 1.5% by weight of mixed hydrophobic surfactant proteins (SP)-B/C purified from calf lung surfactant extract (CLSE). Pure DEPN-8 had better adsorption(More)
Since their inception, tetracycline (Tet)-inducible systems have become the method of choice for transgenic research. The Tet-Off systems have a number of advantages, including robust target induction using a relatively benign effector molecule. However, use of the Tet-On system has been fraught with difficulties, including high background expression in the(More)
In this report we describe three monoclonal antibodies which detect prepatterning events preceding the appearance of visible tips in Polysphondylium pallidum whorls. A spatial and temporal analysis of the antigens against which these antibodies are directed reveals that the radial distribution of arms within whorls has its origins in an initial global(More)
High-resolution X-ray microtomography was used to get deeper insight into the underlying mass transport mechanisms controlling drug release from coated pellets. Sugar starter cores were layered with propranolol HCl and subsequently coated with Kollicoat SR, plasticized with 10% TEC. Importantly, synchrotron X-ray computed microtomography (SR-μCT) allowed(More)
During morphogenesis in the slime mold Polysphondylium pallidum cell masses are periodically pinched off from the base of the developing sorogen. These masses round up and differentiate into secondary sorogens, which become radially ordered arrays of secondary fruiting bodies called whorls. Here we describe the morphogenesis of P. pallidum and characterize(More)
The branches in Polysphondylium pallidum whorls are arranged in a radial pattern. We have used a pattern-specific monoclonal antibody to study branch formation and characterize the origin of this pattern. A quantitative spatial analysis of antibody staining reveals that the branching pattern arises from a random distribution. This distribution passes(More)