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Real-time reverse transcription followed by polymerase chain reaction (RT-PCR) is the most suitable method for the detection and quantification of mRNA. It offers high sensitivity, good reproducibility and a wide quantification range. Today, relative expression is increasingly used, where the expression of a target gene is standardised by a non-regulated(More)
BACKGROUND Altering the macronutrient composition of the diet influences hunger and satiety. Studies have compared high- and low-protein diets, but there are few data on carbohydrate content and ketosis on motivation to eat and ad libitum intake. OBJECTIVE We aimed to compare the hunger, appetite, and weight-loss responses to a high-protein,(More)
Selenium (Se), a micronutrient essential for human health, is incorporated into at least 25 selenoproteins including selenoprotein P (SePP), which transports Se within the body. This research identified two single nucleotide polymorphisms (SNPs) in the SePP gene, one in the coding region (position 24731, causing an Ala to Thr change) and one in the(More)
BACKGROUND It is not clear how decreased activity quantitatively affects energy balance (EB) in subjects feeding ad libitum. OBJECTIVE We assessed the effect of an imposed sedentary routine on appetite, energy intake (EI), EB, and nutrient balance in lean men for 7 d. DESIGN Six men with a mean (+/-SD) age of 23.0 +/- 2.3 y, weight of 69.2 +/- 11.4 kg,(More)
OBJECTIVE The study aimed to establish the effects of age, gender and age-gender interactions on BMI-% fat relationships over a wide range of BMI and age. It also aimed to examine controversies regarding linear or curvilinear BMI-% fat relationships. METHODS Body composition was measured using validated bio-impedance equipment (Bodystat) in a large(More)
We assessed the effect of no exercise (Nex; control) and high exercise level (Hex; approximately 4 MJ/day) and two dietary manipulations [a high-fat diet (HF; 50% of energy, 700 kJ/100 g) and low-fat diet (LF; 20% of energy, 300 kJ/100 g)] on compensatory changes in energy intake (EI) and energy expenditure (EE) over 7-day periods. Eight lean men were each(More)
OBJECTIVE To reassess the validity of the Schofield equations for predicting basal metabolic rate (BMR) in the obese. DATA The data collection compiled from many studies by Schofield in 1985. METHODS Nonparametric curve fitting and comparison of groups. RESULTS BMR increases more slowly at heavier weights in men (above about 75 kg) and women (above(More)
Two studies have examined the effect on energy intake and macronutrient selection of increasing increments of mandatory high-fat or high-sugar snacks into the diet in men. The present study used a within-subject, repeated-measures design. In each experiment, six lean and six overweight, unrestrained men were each studied over three 7 d treatment periods,(More)
BACKGROUND Epigenetic regulation of imprinted genes and transposable elements has been implicated in human disease and may be affected by maternal diet. OBJECTIVE The objective was to determine the effect on offspring epigenetic status of nutritional and genetic factors that influence folate exposure in pregnancy. DESIGN We measured folate intake from(More)
Gastrointestinal (GI) tract leucine metabolism was measured in 6- to 9-mo-old lambs subjected to trickle infection with Trichostrongylus colubriformis larvae and in separate animals that were not infected. Animals prepared with a jejunal catheter and with indwelling catheters into the aorta and the portal- (PDV) and mesenteric- (MDV) drained viscera were(More)