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Real-time reverse transcription followed by polymerase chain reaction (RT-PCR) is the most suitable method for the detection and quantification of mRNA. It offers high sensitivity, good reproducibility and a wide quantification range. Today, relative expression is increasingly used, where the expression of a target gene is standardised by a non-regulated(More)
Selenium (Se), a micronutrient essential for human health, is incorporated into at least 25 selenoproteins including selenoprotein P (SePP), which transports Se within the body. This research identified two single nucleotide polymorphisms (SNPs) in the SePP gene, one in the coding region (position 24731, causing an Ala to Thr change) and one in the(More)
Particles are random sets whose position and orientation are irrelevant. They are traditionally handled by calculating summaries of shape, such as compactness and elongation, or by deening landmarks, whose positions are then subject to statistical analysis. It would be advantageous in many applications if shape variability could be addressed without the(More)
To date, no study has directly and simultaneously measured the discrepancy between what people actually eat and what they report eating under observation in the context of energy balance (EB). The present study aimed to objectively measure the 'extent' and 'nature' of misreporting of dietary intakes under conditions in which EB and feeding behaviour were(More)
The effects of incremental exercise on appetite, energy intake (EI), expenditure (EE) and balance (EB) in lean men and women were examined. Six men (age 29.7 (SD 5.9) years, weight 75.2 (SD 15.3) kg, height 1.75 (SD 0.11) m) and six women (age 24.7 (SD 5.9) years, weight 66.7 (SD 9.10) kg, height 1.70 (SD 0.09) m) were each studied three times during a 16 d(More)
The most lethal aspects of gastric adenocarcinoma (GA) are its invasive and metastatic properties. This aggressive phenotype remains poorly understood. We have recently identified neuroepithelial cell transforming gene 1 (NET1), a guanine exchange factor (GEF), as a novel GA-associated gene. Neuroepithelial cell transforming gene 1 expression is enhanced in(More)
In crop variety registration, visual scores of a plant's appearance are widely and routinely used to establish variety differences. This makes it an ideal candidate for automating through machine vision. This paper describes the outline of a planned system for searching for matches between images from new candidate varieties and those stored in a database(More)
OBJECTIVE The purpose of the study was to examine the effect of energy density and food weight (volume) on subsequent intake. DESIGN Sixteen lean men were each studied four times during a 2-d protocol at the Rowett's Human Nutrition Unit. On day 1, subjects were fed a mandatory diet at 1.6 x resting metabolic rate (RMR). On day 2, during the mandatory(More)
BACKGROUND Proteomic technologies applied for profiling human biofluids and blood cells are considered to reveal new biomarkers of exposure or provide insights into novel mechanisms of adaptation. METHODS Both a non-targeted (classical 2D-electrophoresis combined with mass spectrometry) as well as a targeted proteomic approach (multiplex immunoassay) were(More)