Graham Vaughan Lees

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Mechanically dissociated neuronal somata from the thoracic ganglia of Locusta migratoria and Schistocerca gregaria were viable in vitro for hours and were current- and voltage-clamped to record the responses evoked by brief pressure applications of gamma-aminobutyric acid (GABA) in the presence of various modulators. The application of GABA and muscimol,(More)
Cultured neurones from the cockroach, Periplaneta americana, have been used to investigate putative acetylcholine receptors. Ligand-binding experiments revealed that these neurones possessed an alpha-bungarotoxin binding site that was saturable, had an apparent affinity constant of 3.51 nM and was predominantly nicotinic in nature. An individual culture of(More)
The physiological effects of dihydroavermectin B1 on insect central neurones have been investigated using a culture system derived from the brains of embryonic cockroaches. In these neuronal cultures 60% of the cells respond to the application of gamma-aminobutyric acid (GABA) with a conductance increase; these responses are blocked by picrotoxin but not by(More)
SUMMARY Gamma-aminobutyric acid (GABA) at 10~ 4 moll~' inhibited spontaneous activity and produced conductance changes in 60% of cultured cockroach neurones tested. The reversal potential for the GABA-evoked response was between —65 mV and —75 mV. Under whole-cell voltage-clamp conditions, with 114mmoll~' potassium chloride in the electrode, the reversal(More)
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