Graham Palmer

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The increasing availability of instruments capable of following absorbance or fluorescence changes in the millisecond time range has led in recent years to a large number of studies of ligandprotein interactions. Of particular interest to the enzymologist are cases where partial reactions in the catalytic cycle may be followed. For example, the reaction of(More)
In the absence of any external electron donor, the "peroxy" intermediate of cytochrome c oxidase (CcO-607) is converted to the ferryl form (CcO-580) and subsequently to oxidized enzyme. The rate of conversion of CcO-607 to the CcO-580 form is pH dependent between pH 3.0 and pH 7.6. A plot of the logarithm of the rate constant for this conversion is a linear(More)
De-Perimeterisation is a new term to describe phenomena becoming common in modern ICT environments. It refers to the erosion of the hard-shell model used to describe the structure of traditional information security implementations while capturing the growing requirement for perimeters to be breached in order to facilitate commerce and collaboration. The(More)
OBJECTIVE Targeted next generation sequencing (NGS) was evaluated for its ability to identify unanticipated targetable genomic alterations (GA) for patients with relapsed ovarian epithelial carcinoma (OC). METHODS DNA sequencing was performed for 3320 exons of 182 cancer-related genes and 37 introns of 14 genes frequently rearranged in cancer on indexed,(More)
AIMS Small cell lung cancer (SCLC) carries a poor prognosis, and the systemic therapies currently used as treatments are only modestly effective, as demonstrated by a low 5-year survival at only ∼5%. In this retrospective collected from March 2013 to study, we performed comprehensive genomic profiling of 98 small cell undifferentiated lung cancer (SCLC)(More)
The changes in optical and EPR spectra observed during the anaerobic titration of xanthine oxidase with dithionite can be accounted for by simple oxidation-reduction equilibria involving Mo(VI) u MO(V) H Mo(IV); FAD cf FADH. ++ FADH2, and the l-electron reduction of each of the two ironsulfur centers. The equilibrium constants so obtained permit the(More)
The "peroxy" intermediate (P form) of bovine cytochrome c oxidase was prepared by reaction of the two-electron reduced mixed-valence CO complex with (18)O(2) after photolytic removal of CO. The water present in the reaction mixture was recovered and analyzed for (18)O enrichment by mass spectrometry. It was found that approximately one oxygen atom ((18)O)(More)