Graham D. Bonnett

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BACKGROUND The ability of sugarcane to accumulate high concentrations of sucrose in its culm requires adaptation to maintain cellular function under the high solute load. We have investigated the expression of 51 genes implicated in abiotic stress to determine their expression in the context of sucrose accumulation by studying mature and immature culm(More)
Sucrose phosphate synthase (SPS) is a key enzyme in the production of sucrose. Five SPS gene families have been identified in monocotyledonous plants including sugarcane. Using SPS family-specific primers to four of the five families (we had previously characterised the fifth gene family), an approximately 400-nt region was amplified from the parents of a(More)
Sugarcane is a vegetatively propagated crop and hence the production of seed and its fate in the environment has not been studied. The recent development of genetically modified sugarcane, with the aim of commercial production, requires a research effort to understand sugarcane reproductive biology. This study contributes to this understanding by defining(More)
Fire can be an important mechanism by which invasive grasses maintain their dominance in introduced habitats. In April 2009 a dense stand of Saccharum spontaneum in the Panama Canal Watershed burned as a result of an anthropogenic fire. Regrowth was monitored throughout the 2009 growing season and compared to a nearby unburned stand with a similar(More)
Sugarcane genetic improvement is hampered by the inconsistent flowering of clones. This not only limits choice of parents but also restricts the ability to transfer new traits including GM traits. Further, it is believed to be easier to generate transgenic plants from callus derived from plants induced to flower. Consequently, being able to induce flowering(More)
The recent development of genetically modified sugarcane, with the aim of commercial production, requires an understanding of the potential risks of increased weediness of sugarcane as a result of spread and persistence of volunteer sugarcane. As sugarcane is propagated vegetatively from pieces of stalk and the seed plays no part in the production cycle,(More)
Sugarcane (Saccharum spp. hybrids) accumulates high concentrations of sucrose in its mature stalk and a considerable portion of carbohydrate metabolism is also devoted to cell wall synthesis and fibre production. We examined tissue-specific expression patterns to explore the spatial deployment of pathways responsible for sucrose accumulation and fibre(More)
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