Grace S. -F. Lee

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A barley aleurone cDNA library was screened using 32P-labeled cDNA prepared by reverse transcription of mRNA from aleurone layers treated in the presence or absence of gibberellic acid (GA). Besides α-amylase cDNA clones, another set of clones representing an abundant mRNA in aleurone cells was identified. Messenger RNA hybrid-selected by a prototype clone(More)
A forward mutation system has been developed to obtain rapidly clonable mutants at the URA3 locus in yeast by means of selection for 5-fluoroorotic acid resistance. We have used this system to determine base changes in 35 spontaneous and 34 ultraviolet radiation-induced ura3 base substitution mutants. Other mutants (frameshift, deletion, duplication,(More)
Mutation of the inositol polyphosphate 5-phosphatase OCRL1 results in two disorders in humans, namely Lowe syndrome (characterized by ocular, nervous system, and renal defects) and type 2 Dent disease (in which only the renal symptoms are evident). The disease mechanisms of these syndromes are poorly understood. Here we identify two novel OCRL1-binding(More)
Amyloid precursor protein (APP) cleaving enzyme (BACE) is the enzyme responsible for beta-site cleavage of APP, leading to the formation of the amyloid-beta peptide that is thought to be pathogenic in Alzheimer's disease (AD). Hence, BACE is an attractive pharmacological target, and numerous research groups have begun searching for potent and selective(More)
Runx2 is one of the most important transcription factors directing the osteogenesis of mesenchymal stem cells and osteoblastic functions. It is likely that the factors controlling Runx2 expression would trigger the early steps of osteoblast differentiation. By using a reporter gene assay for 4.5 kb Runx2 promoter, it was found that the first 305 bp of Runx2(More)
Starch gel electrophoretic methods for the demonstration of 3 placental enzymes, glucose dehydrogenase, beta-glucoronidase, and N-acetyl-beta-glucosaminidase, are reported. With these methods, the zymograms of these 3 enzymes from placental extracts, gestational and nongestational whole-blood extracts and sera, and sera from women taking an oral(More)