Grace Kronauer

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Ten strains of hepatitis A virus (HAV) originating from far distant geographical locations were adapted to growth in PLC/PRF/5 (human hepatoma derived and/or MRC-5 (human embryonic lung) cells. In the course of primary adaptation some of these strains exhibited a predilection for distinct cultural conditions such as type of host cell and temperature of(More)
The stabilities of hepatitis A virus (HAV) and of poliovirus type 2 were compared under strictly controlled, identical conditions of pH value, temperature, and salt concentration. Although the resistance of the viruses proved to be the same from pH 3 to 11, the temperature at which 50% of poliovirus particles became disintegrated during heating at pH 7.0(More)
Canine parvovirus (CPV), feline panleukopenia virus (FPLV) and mink enteritis virus (MEV) were compared serologically, by determination of their host range in cell cultures, as well as by restriction enzyme analysis. Maps of the virus genomes were established using seven different restriction enzymes cutting at a total of 56 sites. MEV and FPLV gave maps(More)
Plaque formation with representative strains of feline panleukopenia virus (FPV) has been obtained using a permanent line of feline kidney cells under agarose overlay. FPV-infected cells appear as white plaques after neutral red staining. Plaque size is determined by the extent of cell division in the infected monolayer. FPV assay by the plaque procedure is(More)
1. Das Haemagglutinin des Gelbfiebervirus kann in infizierten menschlichen Explantaten sowohl im Gewebe (durch alkalische Extraktion mit Boratpuffer) als auch in der flüssigen Kulturphase (nach der Eliminierung unspezifischer Inhibitoren mit Freon) quantitativ nachgewiesen werden. 2. Im explantierten Gewebe ist Haemagglutinin bereits vor dem cytopathogenen(More)
Certain human tumor cell lines after treatment of tissue monolayers with an alkaline borate buffer yielded a particular hemagglutinin. Based on its properties (hemagglutinating activity, behaviour in tissue cultures, stability and sedimentation velocity) the isolated hemagglutinin seems to be different from other known hemagglutinating viruses. A possible(More)
1. In Erweiterung vorgängiger Versuche wurde der Nachweis geleistet, daβ der klassische Geflügelpeststamm „Brescia“ in Explantaten von menschlichem Normalgewebe (Amnion, Niere) und menschlichen Tumorzellen (HeLa, KB) in Dauerpassagen gezüchtet werden kann. 2. Das bereits früher beschriebene Phänomen der Virusabwandlung bzw. Isolierung von apathogenen(More)
Aus menschlichen Explantaten (Amnion-, HeLa-, KB- Zellstämme) gewonnene Varianten des klassischen und atypischen Geflügelpestvirus zeigten bei der Immunisierung von Hühnern die folgenden Eigenschaften: 1. einen hohen Grad von Mitigierung, der die Verimpfung gröβter Infektionsdosen bei jeder Art von Applikation ohne nennenswerte Schädigung der Versuchstiere(More)