Grażyna Bielecka

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The analytical procedure of thiamine quantification in premixtures and compound feedingstuffs by HPLC method is presented. Thiamine was extracted from feed samples with 0.1M hydrochloric acid at 100°C for 30 min. In the case of compound feedingstuffs, 10% Taka-diastase solution was added to the samples, and then the samples were incubated at 37°C for 17 h.(More)
Feed additives have been widely used in animal nutrition. Recommendations concerning using feed additives, their categories and the description of requirements related to such additives can be found in the regulation (EU) No 1831/2003 [1]. Detailed regulations oblige the entities launching feed additives on the market to specify the methods used to analyse(More)
The analytical procedure of canthaxanthine quantification in premixes and compound feed by the HPLC method was presented. The principle of this method is based on the hydrolysis of powdered formulation of canthaxanthine with trypsin and pepsin in a water solution of ammonia and its purification in an aluminium oxide filled column. The canthaxantine content(More)
The analytical procedure of riboflavin quantification in premixtures and compound feedingstuffs by high performance liquid chromatography (HPLC) analysis is presented. Riboflavin was extracted from feed sample in autoclave with 0.1M sulphuric acid. The ester bonds with phosphoric acid were hydrolysed by the Taka-diastase enzyme. Riboflavin content was(More)
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