Learn More
Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) are involved in tumour progression and metastasis. In this study, we investigated the in vitro and in vivo expression patterns of MMP-1, MMP-2, MMP-3, MMP-9, TIMP-1 and TIMP-2 mRNA and protein in a previously described human melanoma xenograft model. This model consists of eight human(More)
In recent years, large discrepancies were described in the success rate of the tyrosinase reverse transcription polymerase chain reaction (RT-PCR) for detecting melanoma cells in the peripheral blood of melanoma patients. We present a quality control study in which we analysed the reproducibility of detection of tyrosinase and MART-1 transcripts in 106(More)
We recently found that the proteins of the proteolytic system of plas-minogen activation emerge in late stages of melanocytic tumor progres sion. A large body of evidence suggests a role for two proteins, the low-density lipoprotein receptor-related protein (LRP)/a2-macroglobulin receptor and its receptor-associated protein (KAP), in the ¡nternalization of(More)
BACKGROUND Cryoablation has been used successfully for the local treatment of several cancers. Besides local destruction, a systemic antitumor response has been postulated after cryoablation of tumor tissue. In this study we evaluate the possible systemic antitumor response induced by cryodestruction of tumor tissue in two mouse tumor models. METHODS Mice(More)
Dendritic cells are the professional antigen-presenting cells of the immune system. To induce an effective immune response, these cells should not only express high levels of MHC and costimulatory molecules but also migrate into the lymph nodes to interact with naı¨ve T cells. Here, we demonstrate that in vitro-generated mature, but not immature dendritic(More)
Heparin, located in mast cells and baso-philic granulocytes, is widely used as an anticoagulant. It belongs to a class of linear polysaccharides called glycosami-noglycans (GAGs). Using phage display technology, we have selected 19 unique human antiheparin antibodies. Some anti-bodies react almost exclusively with hep-arin, others also react with the(More)
Enzyme-linked immunosorbent assay (ELISA) methods and immunohistochemistry (IHC) are techniques that provide information on protein expression in tissue samples. Both methods have been used to investigate the impact of the plasminogen activation (PA) system in cancer. In the present paper we first compared the expression levels of uPA, tPA, PAI-1 and uPAR(More)
The detection of minimal amounts of melanoma cells by tyrosinase reverse transcription polymerase chain reaction (RT-PCR) is seriously hampered by false negative reports in blood of melanoma patients with disseminated melanoma. Therefore, additional assays which make use of multiple melanoma markers are needed. It has been shown that introduction of(More)
The expression of cytokeratins (CKs) in human lung cancer was studied using chain-specific monoclonal antibodies were detected in all tumors, while CK 4 was found in high concentrations in some ACs. CK 10 and 13 were completely absent, or only present in low numbers of cells. Small cell lung cancers (SCLCs) and lung carcinoids contained CK 18 and sometimes(More)
  • 1