Gloria Aguilar Cruz

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We have increased up to 65-fold the avidity of BR96, a mAb recognizing Lewis Y (Le(y))-related Ags expressed on the surface of many human carcinomas. Libraries of mutations in the complementarity-determining regions (CDRs) of BR96 were constructed in an M13 phage Fab expression vector by codon-based mutagenesis, a method that efficiently introduces large(More)
We have used a combinatorial mutagenesis strategy to humanize BR96, a monoclonal antibody that binds to the Lewis Y class of tumor antigens. This approach allows simultaneous assessment of hundreds of humanized variable regions to identify the molecules that best preserve affinity, thus overcoming the major drawback of current humanization procedures, the(More)
A graph is said to be planar if it can be drawn on the plane with vertices as different points and edges as continuous curves that only intersect its vertices. An embedding of a graph is said to be primitive if its edges are primitive segments. A recent conjecture is that all planar graphs with n vertices have a primitive embedding in a square grid of side(More)
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