Glen Newman

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1. In addition to proteins that are insoluble in organic solvents, lamellated bodies isolated from pig lung and surfactant prepared from bronchopulmonary lavage fluid contain another group of proteins that are extracted together with lipid into the organic phase. 2. These hydrophobic proteins constitute about 40% of the total protein of lamellated bodies(More)
Platelet-activating factor (PAF) was estimated in extracts of human follicular and amniotic fluids using a commercially available 125I-radioimmunoassay. Levels obtained before and after purification of the extracts by thin-layer chromatography were similar and PAF could be estimated with high accuracy and reproducibility over a wide dilution range. PAF(More)
Fetal surfactant stimulates the synthesis of prostaglandins by slices of human amnion at term and by a human amnion cell line, and these effects are partly dependent upon surfactant apoproteins. In this paper, methods are described for the purification of surfactant from human amniotic fluid and from post-mortem human lung. A procedure is described for the(More)
Discs of amnion and choriodecidua prepared from women delivered at term were incubated with and without surfactant prepared from human amniotic fluid and the output of prostaglandin E (PGE) was estimated by radioimmunoassay. Surfactant stimulated the release of PGE in both tissues. The stimulatory effect was characterized further using cultured human amnion(More)
The phospholipids of human fetal pulmonary surfactant prepared from term amniotic fluid contained arachidonic acid and its utilization for prostaglandin synthesis by amnion cells has been investigated. Cells were incubated with surfactant labelled with L-alpha-1-palmitoyl-2-[1-14C]arachidonylphosphatidylcholine. The uptake of radioactivity into amniocyte(More)
Phospholipase A2 activity (EC 3.1.1.4) was estimated in low-speed supernatants of human placenta by measuring the release of arachidonic acid from phosphatidylcholine, 1-stearoyl-2-[3H]arachidonyl and other phospholipids under alkaline conditions (pH 8). Activity was calcium dependent and followed Michaelis-Menten kinetics with an apparent Km of 0.19 (SD(More)
The effects of bronchopulmonary lavage with isotonic NaCl have been investigated in the hamster, and methods are described for separating pulmonary surfactant from cells in lavage fluid and for the quantitative isolation of surfactant from lung. It has been shown that hamsters almost invariably survived a lavage which consisted of ten successive washes each(More)
Discs of human amnion prepared from nine women delivered by elective caesarean section at term were incubated with and without purified human amniotic fluid surfactant (9 micrograms lipid P/ml), and the output of prostaglandin E (PGE) was estimated by radioimmunoassay. Surfactant stimulated the release of PGE from 3.8 (SD 2.9) to 5.4 (SD 2.5) pmol/mg dry(More)
BACKGROUND Obesity is an increasing epidemic that can complicate the treatment of simple injuries and can increase health care costs. The purpose of the present study was to determine whether obesity is a factor in the utilization of inpatient physical therapy services and length of stay following a traumatic lower leg fracture. METHODS A retrospective(More)
I . A semi-synthetic vitamin B,,-deficient diet, based on soya flour, is described. When supplemented with cydnocobalamin the diet appeared to be adequate for growth and reproduction in the rat. 2. Compared with their litter-mates on the supplemented diet, rats fed on the deficient diet from weaning showed reduced levels of vitamin Biz activity in the(More)