Gleb D Churbanov

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Three types of morphologically and functionally distinct taste cells operate in the mammalian taste bud. We demonstrate here the expression of two G-protein-coupled receptors from the family C, CASR and GPRC6A, in the taste tissue and identify transcripts for both receptors in type I cells, no transcripts in type II cells and only CASR transcripts in type(More)
The extracellular Ca(2+)-sensing receptor (CASR) is a promiscuous G-protein-coupled receptor closely related to the taste receptors T1R1-T1R3. Here we analyzed the possibility that apart from being stimulated by external Ca(2+) and amino acids, the substances effective as tastants, CASR might serve as a receptor for other sapid compounds. CASR was(More)
Taste cells of type I express the polymodal receptor CASR previously found in a heterologous system to recognize bitter denatonium as a ligand. Here we studied responsiveness of the type I cells to a variety of sapid compounds using Ca2+ imaging. Taste cells were isolated from mouse CV papillae and loaded with Ca2+ dye Fluo-4. Type I cells were identified(More)
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