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Paracrine Mechanisms of Mesenchymal Stem Cells in Tissue Repair.
TLDR
It is proposed that the functional benefits observed after MSC transplantation in experimental models of tissue injury might be related to the secretion of soluble factors acting in a paracrine fashion, and this hypothesis is supported by pre-clinical studies demonstrating equal or even improved organ function upon infusion of MSC-derived conditioned medium (MSC-CM) compared with M SC transplantation.
Conditioned Medium From Human Amniotic Mesenchymal Stromal Cells Limits Infarct Size and Enhances Angiogenesis
TLDR
The results strongly support the concept that the administration of hAMC‐CM favors the repair process after acute myocardial infarction.
Protocols for in vitro Differentiation of Human Mesenchymal Stem Cells into Osteogenic, Chondrogenic and Adipogenic Lineages.
TLDR
Standard protocols for the differentiation of BM-MSC into the osteogenic, chondrogenic, and adipogenic lineages are described.
Combination of miRNA499 and miRNA133 Exerts a Synergic Effect on Cardiac Differentiation
TLDR
The results show that the areas spontaneously contracting possess electrophysiological and pharmacological characteristics compatible with true cardiac excitation‐contraction coupling, and the translational relevance was reinforced by the demonstration that the over‐expression of miRNA499 and miRNA133 was also able to induce the differentiation of human mesenchymal stromal cells toward the cardiac lineage.
Proteotoxicity in cardiac amyloidosis: amyloidogenic light chains affect the levels of intracellular proteins in human heart cells
TLDR
The results support and expand the concept that soluble amyloidogenic cardiotropic LCs exert toxic effects on cardiac cells and affect proteins involved in cytoskeletal organization, protein synthesis and quality control, mitochondrial activity and metabolism, signal transduction and molecular trafficking.
Novel mitochondrial protein interactors of immunoglobulin light chains causing heart amyloidosis
TLDR
It is shown that cardiotoxic LCs establish nonphysiologic protein‐protein contacts in human cardiac cells, offering new clues on the pathogenesis of AL cardiomyopathy.
Testing the Paracrine Properties of Human Mesenchymal Stem Cells Using Conditioned Medium.
TLDR
In this chapter, a straightforward method to prepare MSC-derived CM is described and some in vitro assays useful for testing the cytoprotective, angiogenic, and regenerative activity of CM are summarized.
Overexpression of growth factors to improve cardiac differentiation of human mesenchymal stem cells derived from the amniotic membrane
TLDR
It is shown that the overexpression of either IGF-I, or BMP2, or TGFβ1 or B MP2, significantly increases the differentiation efficiency of A-MSC into CMC, which seems to be the most relevant cardiac differentiation triggers.
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