Giulio Calzaferri

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Total As content may be determined in blood and urine by means of an AAS method that involves reduction of As to its volatile hydride and ashing at 600 degrees C with MgO and Mg (NO3)2. Separation of inorganic As (InAs), monomethylarsonic acid (MMA) and dimethylarsinic acid (DMAA) by ion-exchange chromatography, followed by direct AAS analysis, allows the(More)
Aluminium in urine (AlU) and in plasma (AlP) was determined in seven subjects occupationally exposed to environmental concentrations of aluminium below or equal to the TWA (5 mg/m3). The AlU levels in these workers were markedly higher than those found in the control group. The levels of the indicator were definitely higher at the end of the shift than at(More)
PURPOSE Urinary extracellular vesicles (UEVs) are a novel source for disease biomarker discovery. However, Tamm-Horsfall protein (THP) is still a challenge for proteomic analysis since it can inhibit detection of low-abundance proteins. Here, we introduce a new approach that does not involve an ultracentrifugation step to enrich vesicles and that reduces(More)
We measured serum tartrate-resistant acid phosphatase (TRAP) activity in 120 healthy subjects and 35 patients with multiple myeloma as well as urinary hydroxyproline excretion in the myeloma patients. Young subjects (0-18 years) showed higher TRAP levels (ANOVA p less than 0.01) compared with the other age classes due to the more active bone remodelling(More)
Urinary extracellular vesicles provide a novel source for valuable biomarkers for kidney and urogenital diseases: Current isolation protocols include laborious, sequential centrifugation steps which hampers their widespread research and clinical use. Furthermore, large individual urine sample volumes or sizable target cohorts are to be processed (e.g. for(More)
Concentrations of total serum N-acetyl-neuraminic acid, carcinoembryonic antigen, ferritin, lactate dehydrogenase, creatine phosphokinase and total proteins were measured in both tumor drainage blood (axillary vein) and in peripheral blood taken during surgery from 44 breast cancer patients. There were no significant differences in any of the markers(More)
The levels of cadmium in blood (CdB) and in urine (CdU) were measured in 40 women at delivery and in their offspring. The women were not occupationally exposed to cadmium. The CdB levels in the pregnant women were significantly lower than in a control group consisting of 40 subjects of the same sex and age living in the same area. The difference can(More)
The aim of this research has been to develop analytical procedures whereby the various chemical forms of arsenic present in urine can be distinguished and further data on the biotransformation of absorbed arsenic can be acquired. The separation of inorganic arsenic ( InAs ), monomethylarsonic acid ( MMAA ), and dimethylarsinic acid ( DMAA ) in urine was(More)