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High-resolution computed tomographic characteristics in acute farmer's lung and in its follow-up.
High resolution computed tomography (HRCT) scans are increasingly used in hypersensitivity pneumonitis (HP). This study looked at HRCT findings at different phases of farmer's lung (FL), a commonExpand
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Significance of precipitins and asymptomatic lymphocytic alveolitis: a 20‐yr follow-up
What is the significance of serum preciptins and lymphocytic alveolitis in a healthy subject exposed to antigens responsible for hypersensitivity pneumonitis (HP)? This study was done to evaluate theExpand
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Farming practices and the respiratory health risks of swine confinement buildings.
This study investigated whether clean swine confinement buildings (SCB) are less harmful to the respiratory system than older and dirtier facilities. Eight healthy volunteers were exposed for 4 h, atExpand
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Proteomic and transcriptomic analysis of linezolid resistance in Streptococcus pneumoniae.
Linezolid is an oxazolidinone antibiotic that inhibits the initiation of translation. Although resistance to linezolid is an uncommon event, it has been reported in clinical isolates. The genomeExpand
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Analysis of stage-specific expression of basic proteins in Leishmania infantum.
Prior analyses of the proteome of the protozoan parasite Leishmania have underrepresented basic proteins. Here, we applied protein fractionation by isoelectric point (pI) using free-flowExpand
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Mitochondrial Proteomics of Antimony and Miltefosine Resistant Leishmania infantum
Antimony (SbIII) and miltefosine (MIL) are important drugs for the treatment of Leishmania parasite infections. The mitochondrion is likely to play a central role in SbIII and MIL induced cell deathExpand
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Exoproteome dynamics in Leishmania infantum.
UNLABELLED The exoproteome of Leishmania infantum is composed of parasite derived proteins present in the extracellular environment. Although the exoproteome might have a significant role in theExpand
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Analysis of membrane-enriched and high molecular weight proteins in Leishmania infantum promastigotes and axenic amastigotes.
Membrane and high molecular weight (HMW) proteins tend to be underrepresented in proteome analyses. Here, we optimized a protocol designed for the extraction and purification of membranes from theExpand
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Proteomic analysis of metacyclogenesis in Leishmania infantum wild-type and PTR1 null mutant
The parasite Leishmania exhibits a dimorphic life cycle with promastigotes found in the insect vector and amastigotes residing within mammalian macrophages. In the insect, promastigotes undergoExpand
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Separation of basic proteins from Leishmania using a combination of Free flow electrophoresis (FFE) and 2D electrophoresis (2-DE) under basic conditions.
Basic proteins, an important class of proteins in intracellular organisms such as Leishmania, are usually underrepresented on 2D gels. This chapter describes a method combining basic proteinsExpand
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