Gina M. Pighetti

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Streptococcus uberis causes a significant proportion of clinical and subclinical intramammary infections (IMI) in lactating and non-lactating dairy cows. In spite of this, its pathogenesis is incompletely understood. A study was conducted to determine leukocyte and cytokine dynamics during experimentally induced S. uberis mastitis. Five Jersey and five(More)
The ability to identify objectively cows that are more or less susceptible to mastitis has been a long-standing goal. Genetic markers associated with inflammatory responses during mastitis could aid in selection of these cattle. One potential marker is CXCR2, a chemokine receptor required for neutrophil migration to infection sites, which contains single(More)
The objectives of this study were to identify single nucleotide polymorphisms (SNPs) and resulting haplotypes in the bovine CXCR2 gene. A 311-bp segment of the bovine CXCR2 gene was amplified and sequenced. Five SNPs at positions 612, 684, 777, 858, and 861 were expressed in both Holstein and Jersey dairy cattle. Four SNPs resulted in synonymous(More)
The objective was to evaluate the association between the single nucleotide polymorphism at position +735 in the interleukin-8 receptor-α (CXCR1) gene (CXCR1c.735) and disease incidence, milk production, reproductive performance, and survival in Holstein cows. Three-hundred fifty Holstein cows were enrolled. No association was found between CXCR1c.735(More)
Several single nucleotide polymorphisms (SNP) in the bovine CXCR1 gene have been implicated in resistance to mastitis and milk somatic cell counts in several sample populations of Holstein dairy cows. As such, a more thorough understanding of SNP present in and near the bovine CXCR1 gene is needed. This study identified 36 SNP in the coding region and(More)
Bovine mastitis continues to be the most detrimental factor for profitable dairying. Recent research conducted within our laboratory has identified a genetic marker in the CXCR2 gene associated with mastitis susceptibility. The objective of the present study was to evaluate the migratory ability of neutrophils from cows with different CXCR2 +777 genotypes.(More)
The effects of bovine leukosis virus (BLV) on the phenotypic and functional characteristics of peripheral blood mononuclear cells were investigated. Whole blood differentials showed that persistently lymphocytotic (BLV+PL) dairy cattle had more lymphocytes and fewer neutrophils than the aleukemic seropositive (BLV+AL) or seronegative (BLV-) animals. Flow(More)
Once activated, lymphocytes can regulate both specific and nonspecific immune responses. Alterations in lymphocyte function may increase the host's vulnerability to bacterial infections such as mastitis. Susceptibility to mastitis as well as diminished leukocyte functional capabilities have been shown to be influenced by lactational stage. Therefore, the(More)
This study examined the production of tumor necrosis factor (TNF-alpha) by mononuclear cells isolated from peripheral blood and supramammary lymph nodes of periparturient and mid to late lactating dairy cows. Monocyte-enriched cell populations were stimulated with lipopolysaccharide (LPS) and analyzed for TNF-alpha concentrations. Flow cytometric analysis(More)
Interleukin (IL)-8 and its receptors, CXCR1 and CXCR2, are key regulators of inflammation. However, knowledge of these receptors at the genomic level is limiting or absent in cattle. Therefore, our objective was to identify bovine orthologs of human CXCR1 and CXCR2. Alignment of bovine CXCR2 reference mRNA to the bovine genome revealed two regions of(More)