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Laser photocoagulation is currently the standard treatment for sight-threatening diseases worldwide, namely diabetic retinopathy and retinal vein occlusions. The slit lamp biomicroscope is the most commonly used device for this procedure, specially for the treatment of the eye periphery. However, only a small portion of the retina can be visualized through(More)
AIM To examine the differences in manual endothelial cell counting methods in French eye banks and to analyse whether these differences could explain some substantial discrepancies observed in endothelial cell density (ECD) for corneas made available for transplant. METHODS A questionnaire was sent to the 22 eye banks asking for details of the technical(More)
Ocular complications of diabetes, particularly retinopathy, are the major cause of blindness in the working age population in industrialised nations. Laser photocoagulation has shown definite results in reducing visual morbidity with many more likely to benefit if diagnosed early enough. Institution of an efficient screening programme aimed at detecting(More)
PURPOSE Study the clinical and microbiological characteristics and the prognostic factors of post-traumatic endophthalmitis. METHODS Seventeen eyes were included between 2004 and 2010, with clinical and microbiological data collected prospectively. Conventional cultures and panbacterial PCR were performed on aqueous and vitreous samples. RESULTS(More)
PURPOSE Determination of the endothelial cell density (ECD) by eye banks is paramount in donor cornea qualification. Unbiased measurement avoids wastage and grafts with an increased risk of premature failure. Internal calibration of the counting method is essential, but external validation would add an extra stage in the assessment of reliability. In this(More)
PURPOSE To determine the factors influencing endothelial morphometry by using image analysis of corneas stored in organ culture to determine the coefficient of variation (CV) in cell area and percentage of hexagonal cells. METHODS The endothelia of 505 of the 559 corneas consecutively stored at the eye bank were routinely analyzed with Sambacornea(More)
AIMS To test the effectiveness and rapidity of a pair of blood culture bottles in the diagnosis of bacterial and fungal contamination of corneal organ culture media. METHODS 761 microbiological analyses of storage media (Inosol and Exosol, Opsia, Toulouse, France), sampled in all phases of the organ culture at 31 degrees C of 410 consecutive corneas, were(More)
PURPOSE To present an experimental method for determining the viable cell pool of corneal endothelia and its application to assessing predissected endothelial grafts. METHODS The endothelial cell density (ECD) of five pairs of human organ cultured corneas was determined using a standard counting method with a calibrated image analysis system. A thin(More)
Corneal endothelial cells (CECs) are terminally differentiated cells, specialized in regulating corneal hydration and transparency. They are highly polarized flat cells that separate the cornea from the aqueous humor. Their apical surface, in contact with aqueous humor is hexagonal, whereas their basal surface is irregular. We characterized the structure of(More)
BACKGROUND Apoptosis very probably plays a key part in endothelial cell loss during corneal storage in organ culture as well as hypothermic storage. However, the mechanisms underlying endothelial apoptosis are poorly understood. The response of a human corneal endothelial cell (HCEC) line to staurosporine, a known inducer of apoptosis, was investigated to(More)