Gilles Chappuis

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Rabies infection of domestic and wild animals is a serious problem throughout the world. The major disease vector in Europe is the red fox (Vulpes vulpes) and rabies control has focused on vaccinating and/or culling foxes. Culling has not been effective, and the distribution of five vaccine baits is the only appropriate method for the vaccination of wild(More)
The S glycoprotein of feline infectious peritonitis virus (FIPV) has been shown to contain the antigenic sites responsible for eliciting both neutralization and antibody-dependent enhancement. To determine the region of S responsible, overlapping DNA fragments spanning the entire S gene were cloned and expressed as fusion proteins by in vitro transcription(More)
Wildlife vaccination depends on vaccines which can be orally administered by a baiting system. Therefore only two possibilities exist: either the use of attenuated strains of viruses, or recombinant vector viruses. As far as rabies is concerned, the choice of the recombinant vaccinia-rabies virus was made because it was safer and more stable. An in vitro(More)
The primary multiplication site of VVTGgRAB, a recombinant vaccinia virus (VV) expressing the rabies virus G glycoprotein, was studied in comparison with that of the parental VV Copenhagen strain, after oral administration to foxes. Foxes were fed with 10(8) TCID50 of either VVTGgRAB or VV and were sacrificed 12, 24, 48 or 96 h after inoculation. Both(More)
 Feline calicivirus (FCV) is a major oral and respiratory pathogen of cats, able to induce subclinical infection as well as acute disease. It is also characterized by a high degree of antigenic variation. This work sought to address the question of the existence of distinct biotypes of FCV. Eight French, 6 British and 9 American FCV isolates, responsible(More)
In a mass vaccination campaign conducted in Peru in March 1985, 270,000 dogs (65% of the estimated dog population) were vaccinated over the course of 1 month with an inactivated tissue culture vaccine. Since that time no human rabies cases have been reported; in addition, the number of animal rabies cases has declined to only three from a previous mean of(More)
Between 1988 and 1991, 644 serum samples were collected from 480 grizzly bears (Ursus arctos horribilis) and 40 black bears (Ursus americanus) from Alaska, United States of America, and were tested for selected canine viral infections and zoonoses. Antibody prevalence in grizzly bears was 0% for parvovirus, 8.3% (40/480) for distemper, 14% (68/480) for(More)
Feline Viral Rhinotracheitis herpesvirus (Feline Herpesvirus 1; FHV-1) was purified by rate zonal isopycnic centrifugation. Viral nucleocapsids were isolated from the nuclei and purified. Analysis of the purified and radiolabelled viral polypeptides and glycoproteins by gradient SDS polyacrylamide gel electrophoresis revealed that 23 viral proteins and 6(More)
The vaccination of wild animals against rabies has been developed most extensively in Europe. Experiments have demonstrated the efficacy of a vaccinia-rabies recombinant virus administered by the oral route in foxes. The innocuity of this vaccine was tested in the target species as well as in several non-target wild and domestic species. Because of its(More)
The objective of this paper is to review adaptive immunity of young animals using examples from my own experience and from the literature. Trials carried out by us with a modified live and inactivated canine parvovirus vaccine in newborn puppies provide evidence of the immune capacity of these puppies. With regard to transfer of immunity from mother to(More)