Giis H. Schaap

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In 6 volunteers with normal renal function, we investigated the effects of various kinds of protein (soy, lactoprotein and beef) and various amounts of an intravenously administered amino acid solution on glomerular filtration (GFR) and effective renal plasma flow (ERPF). As for the protein-induced changes in renal function, rises in GFR and ERPF were(More)
Animal studies have suggested that 99mTc-mercapto-acetylglycyl-glycyl-glycine (99mTc-MAG3) might be suitable for the determination of the renal plasma flow (RPF) because of its high renal clearance. In this study 131I-orthoiodohippurate (131I-OIH) and 99mTc-MAG3 (labeling always >95%) were administered simultaneously in 11 patients (creatinine clearance(More)
Fifteen patients with chronic renal disease of various origin were investigated after 4 weeks protein restriction, after 4 weeks high protein intake and finally, after 8 weeks dietary supplementation with omega-3 polyunsaturated fatty acids (PUFA's), while adhering to a high protein intake. The highest values for effective renal plasma flow and glomerular(More)
In 30 patients with chronic renal disease, glomerular filtration rate (GFR) and effective renal plasma flow (ERPF) were measured after 4 weeks of protein restriction and subsequently after adhering to a high protein intake for the same period. Twenty-four patients adhered to their advised diets according to the 24-hour urea excretion. These 24 patients(More)
Protein restriction is advocated in patients with chronic renal insufficiency (CRI) in an attempt to slow down further renal function deterioration, with the most obvious effect in patients with chronic glomerulonephritis (GN) and diabetic nephropathy, and much less in other disease entities, such as adult polycystic kidney disease (APKD),(More)
Fluorescence polarization measurements on a FACS II cell sorter were compared with static measurements on a spectrofluorimeter using calibration solutions and Hoechst 33258-labeled cells. For the flow cytometric measurements on the FACS we used a pseudodepolarizer for normalization of the output of the two photomultipliers. The results showed that(More)
Undifferentiated mouse teratocarcinoma cells were cocultivated with differentiated mouse endoderm cells in order to study the possible induction of teratocarcinoma cell differentiation. A difference in DNA content between the two cell types was experimentally introduced to enable the reisolation of the teratocarcinoma cells after cocultivation.(More)