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An algorithm is presented for the calculation of metal ion concentrations from given total metal concentrations (and vice versa) in physiological media containing metal-chelating compounds. In such media, conditions differ from those used for stability constant determination of metal-chelator equilibria; therefore calculated metal ion concentrations are(More)
Experiments were performed to determine whether gill transepithelial calcium fluxes in the freshwater trout (Salmo gairdneri) are passive or require active transport and to characterize the mechanisms involved. A comparison of the in vivo unidirectional flux ratios with the flux ratios calculated according to the transepithelial electrochemical gradients(More)
We describe duplicate leptin genes in zebrafish (Danio rerio) that share merely 24% amino acid identity with each other and only 18% with human leptin. We were also able to retrieve a second leptin gene in medaka (Oryzias latipes). The presence of duplicate leptin genes in these two distantly related teleosts suggests that duplicate leptin genes are a(More)
The effects of cadmium (Cd2+) on calcium (Ca2+) transport in the gills of rainbow trout (Salmo gairdneri) were studied. The gill epithelium of freshwater fish represents a model for a Ca2+-transporting tight epithelium. Unidirectional Ca2+ fluxes in the gills were estimated in an isolated saline-perfused head preparation. Ca2+ influx was not affected when(More)
Leptin is a key factor in the regulation of food intake and is an important factor in the pathophysiology of obesity. However, more than a decade after the discovery of leptin in mouse, information regarding leptin in any nonmammalian species is still scant. We report the identification of duplicate leptin genes in common carp (Cyprinus carpio). The unique(More)
We investigated short-term effects (up to 24 h) of air exposure and confinement, and long-term effects (up to 11 days) of confinement, to elucidate signalling pathways in the stress response of gilthead sea bream Sparus aurata L. Plasma glucose and lactate were taken as indicators of sympathetic activation, and alpha-melanocyte stimulating hormone(More)
C. HAOND1,2, G. FLIK3 AND G. CHARMANTIER1,* 1Laboratoire d’Ecophysiologie des Invertébrés, Université Montpellier II, place Eugène Bataillon, 34095 Montpellier Cedex 5, France, 2Station Méditerranéenne de l’Environnement Littoral, Université Montpellier II, 1 quai de la Dorade, 34200 Sète, France and 3Department of Animal Physiology, University of Nijmegen,(More)
In higher vertebrates, mineralo- (aldosterone) and glucocorticoids (cortisol/corticosterone) exert their multiple actions via specific transcription factors, glucocorticoid (GR) and mineralocorticoid (MR) receptors. Teleostean fishes lack aldosterone and mineral regulatory processes seem under dominant control by cortisol. Despite the absence of the(More)
We used confocal laser scanning microscopy to validate a new and fast co-labelling method to study the distribution of mitochondria-rich (MR) cells in gill filaments and to differentiate between MR cells that are in contact with the water (cells labelled with both DASPMI and ConcanavalinA) and those that are not (DASPMI-positive only). This method was used(More)
Two interferon gamma (IFN-gamma) genes are expressed in immune cells of teleost fish and are potentially implicated in B- and T-lymphocyte responses. IFN-gamma-2 shows structural and functional characteristics to other vertebrate IFN-gamma genes and is associated with T-lymphocyte function. Expression profiling shows IFN-gamma-2 upregulation in(More)