Germain Agnani

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OBJECTIVE To check the efficiency of the cryopreservation procedure by using trypan blue staining of ovarian test fragments to assess the quality of frozen-thawed ovarian tissue. DESIGN Prospective study. SETTING University hospital. PATIENT(S) Patients with polycystic ovary syndrome undergoing laparoscopic ovarian drilling. INTERVENTION(S) Ovarian(More)
OBJECTIVE To report the first case of restoration of ovarian activity and live birth after cryopreserved ovarian tissue autograft in a patient without cancer treated by allogeneic bone marrow transplantation. DESIGN Case report. SETTING University hospital. PATIENT(S) One woman with homozygous sickle cell anemia. INTERVENTION(S) An orthotopic(More)
A total of 304 human pronuclear zygotes and cleaved embryos from the 2- to 9-cell stages, obtained during invitro fertilization attempts, were photographed and retrospectively analysed after transfer for their morphology and size in relation to their developmental stage, using the Imagenia programme of a Biocom 500 image analyser. Morphometric parameters(More)
Septic peri-implantitis is the main clinical complication encountered following the insertion of titanium implants. It may be resistant to conventional antibiotic treatments. Reports in the literature about antibiotic behavior in the presence of titanium remain controversial. They vary from a bacteriostat to a decreased effect of antibiotic. This study(More)
The retention and survival of microorganisms on toothbrushes pose a threat of recontamination for certain patients at risk. In order to measure the influence of brush design and optimize the choice of toothbrush model for complementary studies, the in vitro retention of three microbial species (Porphyromonas gingivalis ATCC 33277, Streptococcus mutans ATCC(More)
Because the down-regulation by progesterone of cystic fibrosis transmembrane conductance regulator (CFTR) expression could be a useful specific marker to define the state of implant receptivity in endometrium, a competitive reverse transcription-polymerase chain reaction (RT-PCR) was developed for quantifying the CFTR mRNA concentration in human endometrial(More)
We investigated the target structures of the epithelial cells responsible for the attachment of Porphyromonas gingivalis by immunocytofluorimetry, enzyme-linked immunosorbent assay, and confocal microscopy. Integrins (beta1, beta3, and alphaV) and E-cadherin played no significant role. Carbohydrates (such as alpha-D-methylglucoside, L-fucose, D- and(More)
The protein profiles of Porphyromonas gingivalis (ATCC 33277 and W83) bound to KB gingival epithelial cells were analyzed by SDS-PAGE and immunoblotting. We found that a 51-kDa component was formed in bacteria that adhered to the KB cells, whereas 26- to 29-kDa bands were less intensive, in contrast to the protein profile of free bacteria. P. gingivalis(More)
We investigated the activity of estrone sulfatase in normal and pathological endometrium. In normal endometrium, the estrone sulfatase activity [pmol E1 ⋅ min−1 ⋅ (mg prot)−1] was 23.13 ± 8.44 (mean ± SD). An increase (p < 0.01) of estrone sulfatase activity (62.81 ± 21.97) was noted in mild endometrial hyperplasia. In focal hyperplasia (when the(More)
Some cases of infertility considered inexplicable have been found to have an immunologic basis. Spermatozoa are highly antigenic cells. When the state of immune tolerance is disrupted, auto-immunization in the man or isoimmunization in the woman can occur. The appearance of antibodies directed against the sperm antigens results in a condition of(More)