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Distinct parental cytoplasms were combined in symmetric tetraploid hybrids of potato by somatic cell fusion. This allowed, in the presence of nearly isogenic nuclear genomes, to estimate the contribution of mitochondrial (mt) and chloroplast (cp) genomes to starch content. Analysis of mt-cp configurations in the complete gene pool of german potato cultivars(More)
The molecular mechanisms underlying cell wall digestibility in maize (Zea mays L.) have been studied in three sets of maize brown-midrib isogenic lines in the genetic background of inbreds 1332 (1332 and 1332 bm3), 5361 (5361 and 5361 bm3), and F2 (F2, F2 bm1, F2 bm2, and F2 bm3). Two complementary approaches, SSH (suppression subtractive hybridization) and(More)
BACKGROUND Cell-wall digestibility is the major target for improving the feeding value of forage maize. An understanding of the molecular basis for cell-wall digestibility is crucial towards breeding of highly digestible maize. RESULTS 865 candidate ESTs for cell-wall digestibility were selected according to the analysis of expression profiles in 1) three(More)
BACKGROUND In traditional vine areas, the production should present a typicity that partly depends on the grapevine variety. Therefore, vine improvement is considered difficult because of the limited choice in the natural variability of the cultivars within the limits of their characteristics. A possibility to circumvent this problem is the use of somatic(More)
BACKGROUND The potyviruses sugarcane mosaic virus (SCMV) and maize dwarf mosaic virus (MDMV) are major pathogens of maize worldwide. Two loci, Scmv1 and Scmv2, have ealier been shown to confer complete resistance to SCMV. Custom-made microarrays containing previously identified SCMV resistance candidate genes and resistance gene analogs were utilised to(More)
BACKGROUND Forage quality of maize is influenced by both the content and structure of lignins in the cell wall. Biosynthesis of monolignols, constituting the complex structure of lignins, is catalyzed by enzymes in the phenylpropanoid pathway. RESULTS In the present study we have amplified partial genomic fragments of six putative phenylpropanoid pathway(More)
The quantification of single nucleotide polymorphism (SNP) allele frequencies in pooled DNA samples using real time PCR is a promising approach for large-scale diagnostics and genotyping. The limits of detection (LOD) and limits of quantification (LOQ) for mutant SNP alleles are of particular importance for determination of the working range, which, in the(More)
BACKGROUND OMT (O-methyltransferase) genes are involved in lignin biosynthesis, which relates to stover cell wall digestibility. Reduced lignin content is an important determinant of both forage quality and ethanol conversion efficiency of maize stover. RESULTS Variation in genomic sequences coding for COMT, CCoAOMT1, and CCoAOMT2 was analyzed in relation(More)
The outcrossing of transgenic oilseed rape in the neighbourhood is of major concern with regard to the actual EU labelling threshold of 0.9% for transgenic contamination in food and feed and the regulations for the coexistence of cultivation of genetically modified crops, conventional and organic farming. In a two-year field trial, the outcrossing(More)
  • Fachbereich Informatik, Norbert Krier, +34 authors Diplomarbeit
  • 1997
In a forest a fox bumps into a little rabbit, and says, Hi, junior, what are you up to ? I'm writing my diplomathesis on how rabbits eat foxes, said the rabbit. Come now, friend rabbit, you know that's impossible! Well, follow me and I'll show you. They both go into the rabbit's dwelling and after a while the rabbit emerges with a satissed expression on his(More)
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