Gerda Horst

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Anti-skeletal muscle antibodies (AMA) were measured in 340 non-thymoma patients with myasthenia gravis (MG) by both an ELISA with citric acid muscle extract and by immunofluorescence assay. Three a-AChR negative but AMA-positive patients were found. The occurrence of AMA was related to the age at onset in patients with an onset of MG beyond 40 years and(More)
In tic disorders, increased seroreactivity against neuronal antigens has been demonstrated, without performing molecular characterization of antigens. Here, unselected patients with a tic disorder were compared with healthy controls, autistic disorder (AD), and obsessive-compulsive disorder (OCD) patients. Seroreactivity against neuroblastoma cells was(More)
OBJECTIVE In systemic lupus erythematosus (SLE) autoantibody production is T cell dependent. For a proper T and B cell interaction, signalling of costimulatory molecules on these cells is necessary. The expression of costimulatory molecules on peripheral blood lymphocytes in patients with SLE in conjunction with disease activity was measured to evaluate(More)
Measuring changes of the T cell receptor (TCR) repertoire is important to many fields of medicine. Flow cytometry is a popular technique to study the TCR repertoire, as it quickly provides insight into the TCR-Vβ usage among well-defined populations of T cells. However, the interpretation of the flow cytometric data remains difficult, and subtle TCR(More)
Healthy aging requires an optimal balance between pro-inflammatory and anti-inflammatory immune responses. Although CD4+ T cells play an essential role in many immune responses, few studies have directly assessed the effect of aging on the balance between effector T (Teff) cells and regulatory T (Treg) cells. Here, we determined if and how aging affects the(More)
Insight into the maintenance of naive T cells is essential to understand defective immune responses in the context of aging and other immune compromised states. In humans, naive CD4+ T cells, in contrast to CD8+ T cells, are remarkably well retained with aging. Here, we show that low-affinity TCR engagement is the main driving force behind the emergence and(More)
OBJECTIVE To compare multiple serum markers for their ability to detect active disease in patients with GCA and in those with PMR. METHODS Twenty-six markers related to immune cells that may be involved in GCA and PMR were determined by ELISA and multiplex assay in the serum of 24 newly diagnosed, untreated GCA/PMR patients, 14 corticosteroid (CS)-treated(More)
The role of anti-muscle antibodies (AMA) in the diagnosis of thymoma in patients with myasthenia gravis (MG) is evaluated. We compared ELISA and Western blot assay for antibodies to citric acid muscle extract (a-CAE) with an immunofluorescence assay (IF). Sera from 234 selected MG patients and 123 controls were tested. There was no essential difference(More)