Learn More
Based on a single-molecule sensitive fluorescence-linked immunosorbent assay, an analytical platform for the detection of lipoarabinomannan (LAM), a lipopolysaccharide marker of tuberculosis, was established that is about 3 orders of magnitude more sensitive than comparable current ELISA assays. No amplification step was required. Also, no particular sample(More)
BACKGROUND Biomarkers of progression from latent Mycobacterium tuberculosis infection to active tuberculosis are needed. We assessed correlations between infection outcome and antibody responses in macaques and humans by high-throughput, proteome-scale serological studies. METHODS Mycobacterium tuberculosis proteome microarrays were probed with serial(More)
Mycolic acids are attractive diagnostic markers for tuberculosis (TB) infection because they are bacteria-derived, contain information about bacterial species, modulate host-pathogen interactions and are chemically inert. Here, we present a novel approach based on mass spectrometry. Quantification of specific precursor → fragment transitions of(More)
The dairy farming systems of Western Europe are based on a simple feeding system composed of grazed and preserved grass, maize silage and concentrates in variable proportions. There is, nevertheless, a great diversity of feeding strategies between dairy farms. Over 5 years, we studied the direct and delayed effects of four feeding strategies on the(More)
We focused our study on the olfactory cells growth on biocompatible polymer films electrodeposited on a silicon microsystem. Several substrates such as polyethyleneimine (PEI), polypropyleneimine (PPI), and polypyrrole (PPy), acting as potentially good candidates for cell culture, were tested in order to allow cells to adhere and proliferate. During their(More)
Quality controls of serological assays have to contain defined amounts of human antibodies specific for the targeted antigen. A prevailing issue for array-based antigen assays is that dozens of antigens are targeted within the same assay. Commonly different patient sera are combined and optimal pools are empirically identified. Here, we report a(More)
BACKGROUND Control and elimination of human African trypanosomiasis (HAT) can be accelerated through the use of diagnostic tests that are more accurate and easier to deploy. The goal of this work was to evaluate the immuno-reactivity of antigens and identify candidates to be considered for development of a simple serological test for the detection of(More)
This study investigates the effects of two feeding systems and two dairy cow breeds on milk yield and composition, physical and sensorial properties of Camembert and Pont-l'Evêque cheeses. The experiment consisted of a 2 × 2 factorial arrangement of treatments. A low energy grass diet with only 15% of concentrate (LowGS) was compared with a high-energy(More)
Age-related changes in motor endplates were studied in the fibularis longus, semitendinosus and longissimus muscles in pigs. They develop from small, compact AChE-active figures in fetuses to typical myoneuronal synapses with a branched subsynaptical structure of AChE-active units. High correlations were found between size of endplates and both myofibre(More)
  • 1