Gerd Höfner

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GABA uptake as well as the distribution of GAT-1, GAT-2 and GAT-3 were investigated in bovine brain membrane fractions. GABA uptake was characterised by kinetic constants and IC50-values for a series of known inhibitors in subcellular fractions of frontal cortex and brainstem obtained by subsequent centrifugations on sucrose gradients. Additionally, the(More)
Cooperative modulation of [3H]MK-801 binding to extensively washed pig cortical brain membranes in the presence of various concentrations of L-glutamate, glycine, spermine, CPP and DCKA was evaluated in association experiments. In saturation experiments [3H]MK-801 labelled a homogeneous population of binding sites with a Kd-value of 1.26 +/- 0.18 nmol 1(-1)(More)
(+/-)-2-(4-Benzylpiperidino)-1-(4-hydroxyphenyl)propan-1-ol ([3H]ifenprodil) binding to a subcellular fraction of porcine hippocampus, which was obtained by centrifugation on a discontinuous sucrose gradient, was investigated with the objective to label selectively the ifenprodil recognition site of native NMDA receptors. Saturation experiments revealed(More)
Binding of [3H]MDL 105,519 to glycine sites on N-methyl-D-aspartate (NMDA) receptors of pig cortical brain membranes was evaluated. [3H]MDL 105,519 labelled a homogeneous population of binding sites with a Kd-value of 3.73 +/- 0.43 nM and a Bmax-value of 3030 +/- 330 fmol/mg protein. Clear correlations between the affinity (Ki) to [3H]MDL 105,519 labelled(More)
Siemens Information Systems Ltd. (SISL) ist das System-und Softwarehaus von Siemens in Indien mit einem Schwerpunkt auf Software Offshore Development. Um sicherzustellen, dass die Herausforderungen beim Aufbau eines Offshore Development Centers gemeistert werden, wurde bei SISL eine systematische Vorgehensweise entwickelt, die zusammen mit den(More)