Gerard James Nuovo

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One difficulty in studying dengue virus (DENV) is the lack of an experimental model that reproduces the human disease. In a previous work, we have shown that BALB/c mice intraperitoneally inoculated with a DENV-2 isolate presented viremia and mild focal areas of liver injuries. In this study, mice were inoculated by the intravenous route and presented(More)
OBJECTIVE To provide a comprehensive immunohistochemical (IHC) map of the temporal expression and tissue distribution of interleukin-1β (IL-1β) through progression of osteoarthritis (OA) in two strains of guinea pigs with varying propensity for spontaneous knee joint disease. METHODS OA-prone Hartley and OA-resistant Strain 13 guinea pigs were collected(More)
Dengue is a public health problem, with several gaps in understanding its pathogenesis. Studies based on human fatal cases are extremely important and may clarify some of these gaps. In this work, we analyzed lesions in different organs of four dengue fatal cases, occurred in Brazil. Tissues were prepared for visualization in optical and electron(More)
BACKGROUND C-X-C motif ligand 1 (CXCL1) is an important chemokine of epithelial origin in rodents and humans. OBJECTIVES To assess in vivo and in vitro the regulation of CXCL1 in equine laminitis. ANIMALS Twenty adult horses. METHODS Real-time quantitative polymerase chain reaction (PCR) was used to assess expression of CXCL1 in samples of laminae,(More)
OBJECTIVE Diminish interleukin-1β (IL-1β) signaling in a model of primary osteoarthritis by RNA interference-based transcript reduction or receptor blockade, and quantify changes incurred on transcript expression of additional mediators. METHODS Knees of Hartley guinea pigs were collected at 120 and 180 days of age following injection with viral vectors(More)
BACKGROUND While there is evidence of laminar leukocyte infiltration in black walnut extract (BWE)-induced laminitis, there is no such evidence for carbohydrate overload (CHO) laminitis. OBJECTIVE To assess presence of leukocytes and signs of epidermal stress/injury in the laminar tissue from horses with CHO-induced laminitis. ANIMALS Twenty-four adult(More)
BACKGROUND Laminar inflammation is one of the earliest events in equine laminitis. Calprotectin (CP), a Damage-Associated Molecular Pattern protein, is overexpressed in inflammatory conditions of human skin. HYPOTHESIS CP is overexpressed in the laminar epidermis of horses with black walnut extract (BWE)-induced laminitis. ANIMALS Twenty adult horses.(More)
REASONS FOR PERFORMING STUDY There is increasing evidence of involvement of inflammatory cells in acute laminitis. OBJECTIVE To immunolocalise monocytes/macrophages and B and T lymphocytes in the laminar tissue of normal horses and those with black walnut extract (BWE)-induced laminitis. METHODS Immunohistochemistry was used in archived laminar tissue(More)
A predominantly sensory peripheral neuropathy is common with human immunodeficiency virus (HIV) infection, but the cause is unknown. Formalin-fixed dorsal root ganglia (DRG), obtained at postmortem from patients with neuropathy and HIV infection and from control subjects, were examined for the presence of HIV DNA by using polymerase chain reaction(More)
BACKGROUND Developing new treatments for glomerulonephritis makes the glomerulus a logical target for gene therapy. Microspheres may lodge in the glomerulus, and replication-deficient recombinant adenoviruses are potent mediators of gene transfer. We postulated that adenoviral-microsphere complexes could result in DNA transfer (transduction) into glomerular(More)