Gerard A. Van Arkel

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Synechocystis 6803 contains at least four cryptic plasmids of 2.27 kb (pUS1, pUS2 and pUS3) and 5.20 kb (pUS4). The 1.70 kb HpaI fragments of the related plasmids pUS2 and pUS3 were cloned into the Apr gene of the E. coli plasmid pACYC177, yielding the Kmr hybrid plasmids pUF12 and pUF3 respectively. pUF3 recombines in Synechocystis 6803 with a 2.27 kb(More)
Two plasmids were constructed consisting of the E. coli vector pACYC184 and the cyanobacterial plasmid pUC1. These recombinants, designated pUC104 and pUC105, can be transformed to E. coli K12 as well as to the cyanobacterium Anacystis nidulans R2 and in both hosts they express their antibiotic markers. pUC104 and pUC105 differ with respect to the location(More)
The gene encoding ferredoxin I (petF1) from the filamentous cyanobacterium Anabaena sp. PCC 7937 (Anabaena variabilis ATCC 29413) was cloned by low stringency hybridization with the ferredoxin cDNA from the higher plant Silene pratensis. The petF1 gene from the unicellular cyanobacterium Synechococcus sp. PCC 7942 (Anacystis nidulans R2) was cloned by low(More)
Eighteen mutant strains of the unicellular cyanobacterium Anacystis nidulans R2 that are unable to assimilate nitrate have been isolated after transposon Tn901 mutagenesis. Characterization of phenotypes and transformation tests have allowed the distinction of five different mutant types. The mutants exhibiting a nitrate reductase-less phenotype were(More)
Complementation experiments with temperature sensitive (ts) and suppressor sensitive (sus) mutants of bacteriophage ϕX174 unambiguously revealed five cistrons on the basis of a clear bipartition of burst sizes. A new group of sus mutants (emeralds) was found, defective in a function essential for growth in Shigella sonnei V64. The complementation between ts(More)
In bacterial genetics some methods have been devised to facilitate the isolation of mutants that cannot be automatically selected, e.g. mutants that lack the ability to synthesize a given growth factor or to ferment a given sugar, and revertants from drug resistance to sensitivity. Of these methods the replica plating technique developed by J. and E. M.(More)
SOON'rli~NS, F. J. C. J. lnteraktie tussen lymfocyt, makrofaag en poliovirus. Een onderzoek naar het mechanisme van de door poliovirus gei'nduceerde renaming der DNA-synthese van de met phytohemagglutinine gestimuleerde, humane, perifere lymfocyt. (Interaction between the lymphocyte, the macrophage and poliovirus. An investigation into the mechanism of the(More)
Bacteriophage ΦX174 replicative from DNA (RF DNA) was formed in the presence of chloramphenicol at a concentration of 40 μg per ml and isolated at 12 and at 55 min. after infection. The component I RF DNA (double stranded covalently closed and twisted form) was separated and divided into a monomer and multimer (dimer) fraction. The frequency of recombinants(More)
Pulse-chase and density shift experiments were performed with ϕX174 infected cells. For density shift experiments the cells were first incubated in [14C]thymine containing medium, and after filtration and washing, transferred into [3H]BrdUrd containing medium. Samples were taken at different times and replicative form (RF) component I DNA was isolated,(More)
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