Gerald W Offer

Learn More
Lengthening of active muscle is an essential feature of animal locomotion, but the molecular processes occurring are incompletely understood. We therefore examined and modelled tension responses to ramp stretches (5% fibre length, L0) over a wide range of velocities (0.1-10 L(0) s(-1)) of tetanized intact rat muscle fibre bundles (L0 approximately 2 mm)(More)
We examined the tension responses to ramp shortening and lengthening over a range of velocities (0.1–5 L 0/s) and at 20°C and 30°C in tetanized intact fibre bundles from a rat fast (flexor hallucis brevis) muscle; fibre length (L 0) was 2.2 mm and sarcomere length ∼2.5 μm. The tension change during ramp releases as well as ramp stretches showed an early(More)
Purified antibodies to the thick filament accessory proteins, C-protein, X-protein and H-protein, have been used to label fibres of three rabbit muscles, psoas (containing mainly fast white fibres), soleus (containing mainly slow red fibres) and plantaris (a muscle of mixed fibre type) and their location has been examined by electron microscopy. These(More)
Water holding in meat has, in the past, been rather poorly understood and has not been explained at all in structural terms. A unifying hypothesis for this phenomenon is that gains or losses of water in meat are due simply to swelling or shrinking of the myofibrils caused by expansion or shrinking of the filament lattice. Myofibrils have been observed by(More)
The stiffness of myosin heads attached to actin is a crucial parameter in determining the kinetics and mechanics of the crossbridge cycle. It has been claimed that the stiffness of myosin heads in the anterior tibialis muscle of the common frog (Rana temporaria) is as high as 3.3 pN/nm, substantially higher than its value in rabbit muscle (~1.7 pN/nm).(More)
We examined the tension change induced by a rapid temperature jump (T-jump) in shortening and lengthening active muscle fibres. Experiments were done on segments of permeabilized single fibres (length (L0) approximately 2 mm, sarcomere length 2.5 microm) from rabbit psoas muscle; [MgATP] was 4.6 mm, pH 7.1, ionic strength 200 mm and temperature(More)