Georgios G. Koliakos

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Regenerative medicine and particular adult stem cells represent an alternative option with several fruitful therapeutic applications in patients suffering from chronic lung diseases including idiopathic pulmonary fibrosis (IPF). Nevertheless, lack of knowledge regarding the origin and the potential of mesenchymal stem cells (MSCs) to differentiate into(More)
OBJECTIVES The application of a novel assay for the direct measurement of prooxidant-antioxidant balance (PAB) in type II diabetes and the evaluation of antioxidant therapy. DESIGN AND METHODS The assay is based on 3,3',5,5'-tetramethylbenzidine and its cation, used as a redox indicator participating in two simultaneous reactions. PAB was determined in(More)
OBJECTIVE Ghrelin and leptin levels are influenced by body fat (BF%), pubertal stage and possibly insulin resistance (IR). The aim of our study was: 1) To compare fasting ghrelin and leptin levels between obese and non-obese, adolescents, 2) to investigate possible correlations of these hormones with BF %, as well as IR. DESIGN Twenty obese insulin(More)
OBJECTIVE To measure serum resistin levels in women with polycystic ovary syndrome (PCOS) and assess possible correlations of resistin to the hormonal and metabolic parameters of the syndrome. DESIGN Clinical study. SETTING University hospital. PATIENT(S) Ninety selected women were classified as follows: group I: 35 anovulatory women with PCOS (body(More)
Oxidative stress plays an important role in the pathobiology of exfoliation syndrome (XFS) and exfoliative glaucoma (XFG). We investigated the prooxidant-antioxidant balance (PAB) in aqueous humour and serum samples of 20 consecutive cases of XFS, 20 of XFG, and 20 age-matched controls, employing a recently described novel assay. The activity of catalase(More)
BACKGROUND Ghrelin is a peptide hormone with orexigenic properties, primarily produced by the stomach. Different changes in fasting ghrelin levels have been reported following bariatric surgery. In this study, we investigate the hypothesis that because ghrelin is mainly produced by the fundus of the stomach, biliopancreatic diversion with sleeve gastrectomy(More)
The effect of adrenaline on normal and obese human Na(+)-H(+) antiport (NHE 1) erythrocyte activity has been studied. Adrenaline increased both intracellular pH (pHi) and Na(+) influx in erythrocyte suspensions. This effect of adrenaline was inhibited by amiloride or EIPA, indicating that adrenaline stimulated NHE 1. Phorbol myristicate ester (PMA), a(More)
BACKGROUND Umbilical cord (UC) mesenchymal cells have the ability to differentiate into various cell types, which make them an easily obtainable source for therapeutic uses. Different approaches have been used to isolate mesenchymal stem cells (MSC). AIM Here, we report a detailed enzymatic method where large number of cells can be efficiently isolated(More)
BACKGROUND/AIMS The exact mechanism that links insulin resistance and vascular disease remains unknown. A significantly increased Na+-H+ exchanger (NHE 1) activity and a different effect of insulin on obese individuals erythrocyte NHE 1 has been suggested. These observations may provide a link between obesity, hyperinsulinemia and vascular disease. (More)
Renal dysfunction in thalassemia patients can be attributed to chronic anemia, and iron overload as well as to desferioxamine (DFO) toxicity. We analyzed the urine of 91 well-maintained homozygous beta-thalassemia patients, with no evidence of renal disease, for early evidence of kidney dysfunction by means of electrophoresis and quantitative biochemical(More)