Georgia N Ukpabi

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Iron is an absolute requirement for nearly all organisms, but most bacterial pathogens are faced with extreme iron-restriction within their host environments. To overcome iron limitation pathogens have evolved precise mechanisms to steal iron from host supplies. Staphylococcus aureus employs the iron-responsive surface determinant (Isd) system as its(More)
Enzymatic haem catabolism by haem oxygenases is conserved from bacteria to humans and proceeds through a common mechanism leading to the formation of iron, carbon monoxide and biliverdin. The first members of a novel class of haem oxygenases were recently identified in Staphylococcus aureus (IsdG and IsdI) and were termed the IsdG-family of haem oxygenases.(More)
IsdG and IsdI are paralogous heme degrading enzymes from the bacterium Staphylococcus aureus. Heme bound by these enzymes is extensively ruffled such that the meso-carbons at the sites of oxidation are distorted toward bound oxygen. In contrast, the canonical heme oxygenase family degrades heme that is bound with minimal distortion. Trp-66 is a conserved(More)
IsdI, a heme-degrading protein from Staphylococcus aureus, binds heme in a manner that distorts the normally planar heme prosthetic group to an extent greater than that observed so far for any other heme-binding protein. To understand better the relationship between this distinct structural characteristic and the functional properties of IsdI,(More)
A major contributor to disease in developing countries is the lack of clean drinking water. Solar water disinfection is a popular method used in such countries and involves elimination of pathogenic bacteria from contaminated water. SODIS requires the storage of contaminated water in clear plastic bottles and exposing the bottles to sunlight for several(More)
Ruffling of metalloporphyrins bound to IsdG and IsdI, two heme degrading enzymes Woo Cheol Lee, Georgia Ukpabi, Michelle L. Reniere, Eric P. Skaar, Michael E. P. Murphy University of British Columbia, Microbiology and Immunology, 2350 Health Sciences Mall, Vancouver, British Columbia, V6T 1Z3, Canada, Vanderbilt University Medical Center, Nashville,(More)
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