George Marsicano

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Expression of various developmentally regulated markers was screened throughout the preimplantation stages of in vitro-derived bovine embryos. This was done by investigating the distribution of several nuclear, cytoplasmic and extracellular proteins by means of immunofluorescence microscopy. While lamin B appeared as a constitutive component of nuclei of(More)
We describe an adaptation of the Dexter technique for obtaining ovine long-term bone marrow cultures able to sustain haemopoiesis in vitro for long periods. Two inocula of bone marrow cells collected at three-five weeks interval, in IMDM supplemented with fetal calf serum (10%), horse serum (10%) and hydrocortisone (5 x 10(-7) M), gave rise to the growth of(More)
In this paper, we describe a learning environment that is being used to engage and empower students' learning in a undergraduate software engineering course. The methodology is a joint of Problem Based Learning (PBL) with Learning by Teaching idea - someone can learn better if he has to teach someone else the same concept. In addition, the students must(More)
One of the most important knowledge area on Software Engineering is Requirements Engineering and know properly related techniques and methods is crucial to a software practitioners. This article aims to present a method adopted to teach Requirements Engineering and Process Modelling on Software Engineering undergraduate course. It was developed a method to(More)
Hyperacute rejection (HAR) occurring after transplantation within phylogenetically distant species is a severe reaction triggered by preexisting xenoreactive antibodies and complement activation, leading to the destruction of the donor organ. Expression of human complement inhibitors in transgenic pig organs prolongs the survival of xenograft in(More)
PURPOSE The major obstacle to successful discordant kidney xenotransplantation is hyperacute rejection (HAR). Complement plays a key role in the induction of HRA, defined by endothelial cell activation, loss of vascular integrity, hemorrhage and thrombosis. The activation of complement is tightly controlled by a number of species-specific regulatory(More)
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