George Kourkoulis

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The widespread availability of programmable site-specific nucleases now enables targeted gene disruption in the zebrafish. In this study, we applied site-specific nucleases to generate zebrafish lines bearing individual mutations in more than 20 genes. We found that mutations in only a small proportion of genes caused defects in embryogenesis. Moreover,(More)
The nuclear protein FOG-1 binds transcription factor GATA-1 to facilitate erythroid and megakaryocytic maturation. However, little is known about the function of FOG-1 during myeloid and lymphoid development or how FOG-1 expression is regulated in any tissue. We used in situ hybridization, gain- and loss-of-function studies in zebrafish to address these(More)
The human erythrocyte travels nearly 300 miles through 170,000 circuits of the circulatory system during its 120day lifespan. This prolonged voyage subjects the red cell membrane to high and varied shear forces, to compression and stretching when traversing sinusoidal capillary beds, to osmotic shrinkage and swelling when passing through the renal medulla,(More)
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